北方寒冷地区优势菌剂除锰、氨氮特性研究和应用
发布时间：2018-11-01 15:35
【摘要】：地下水是我国居民生活用水、工业用水的重要水源。由于水污染未得到有效控制,一些地区的地下水已经受到不同程度的污染,导致氨氮及CODMn超标,加之原生的铁和锰,形成复合型微污染地下水,其中各污染物的去除相互影响,大大增加了地下水处理的难度。因此,同时去除地下水中铁、锰、氨氮和CODMn,已经成为饮用水处理的一个重要课题。本次试验研究通过对新型优势菌剂微生物的低温驯化,改良以往微生物在低温条件下生长缓慢和生物活性低的状态,提高微生物的低温耐受力和生物酶活性来提高氧化效果,并采用地下水除铁除锰常用跌水曝气-陶粒吸附处理工艺,通过对接种低温驯化优势菌剂,强化滤池净化功能,达到有效处理复合型微污染地下水的目的。研究内容包括三部分：一,优势菌剂低温驯化试验；二,响应曲面法确定驯化后优势菌剂最佳生长条件；三,低温驯化后优势菌种处理地下水现场中试。菌种来源为经分离鉴定后具有一定除铁锰、氨氮能力的8株细菌,在提纯常温驯化后,选取5株进行复配试验,按照一定比例制备成优势菌剂,针对北方地区(沈阳为主)地下水水质特点进行低温驯化,使其在环境条件下达到预期处理效果通过对优势菌剂进行一个月的低温驯化试验,驯化后10℃条件下菌剂生长周期明显延长,相比驯化前30℃条件下生长周期明显延长,相比驯化前10℃条件下生长周期明显缩短,很大程度上提高低温驯化后菌剂整体的低温耐受力和氧化性,尽管对比原菌剂常温条件下氧化效果略有不足,但相比驯化前同样温度下的氧化效果明显提高。响应曲面法是一种实验条件寻优的方法,通过试验点设计结果分析软件生成的回归拟合和响应曲面、等高线,方便地求出对应的各因素水平的响应值。由响应曲面法设计试验点实验结果分析,回归方程描述各因子与响应值去除率之间的非线性方程关系是显著的。通过响应曲面法确定优势菌剂的最佳除锰、氨氮条件,所得结果均在试验设计中试验点去除率范围内,实际相似度较高,具有较高的参考价值。采用低温驯化优势菌剂的最佳生长条件,控制曝气量和滤速将菌种接种至水厂现场中试(曝气—过滤工艺)的滤罐中。经过调整期、固定化时期以及稳定期的检测,试验结果表明,跌水曝气-陶粒吸附工艺常规处理通过自然接触氧化和物理吸附能够完成对总铁和有机物的有效去除。超标严重的总锰依靠自然接触氧化和物理吸附完成对总锰的有效去除；而滤速变化并不能保证出水氨氮稳定达标。在投加驯化后的优势菌剂后,经过45天的固定化试验和55天的稳定期运行检测发现,在投加经低温驯化的优势菌剂后,滤料成熟后稳定期运行阶段,通过自然氧化和生物降解作用,能够保证在中等滤速下(2.5m/h)完成对总锰和氨氮的有效去除,保证出水稳定达标。
[Abstract]:Groundwater is an important source of water for domestic and industrial use. Since water pollution has not been effectively controlled, groundwater in some areas has been polluted to varying degrees, resulting in ammonia nitrogen and CODMn exceeding the standard, combined with primary iron and manganese, which form a complex micro-polluted groundwater, in which the removal of pollutants affects each other. It greatly increases the difficulty of groundwater treatment. Therefore, simultaneous removal of iron, manganese, ammonia nitrogen and CODMn, from groundwater has become an important issue in drinking water treatment. In this experiment, the low temperature acclimation of the new dominant microorganism was carried out to improve the oxidation effect by improving the slow growth and low bioactivity of the former microbes under the low temperature condition, and increasing the low temperature tolerance and the biological enzyme activity of the microorganism. In this paper, the technology of aeration and ceramsite adsorption is used to remove iron and manganese from groundwater. By inoculating the dominant bacteria at low temperature and strengthening the purifying function of filter, the purpose of treating compound micro-polluted groundwater effectively is achieved. The research contents include three parts: first, the low temperature acclimation test of dominant bacteria; second, the response surface method to determine the optimal growth conditions of dominant bacteria after acclimation; third, the field pilot test of treatment of groundwater with dominant bacteria after low temperature acclimation. After purification and domestication at room temperature, 5 strains of bacteria were selected for compound test, and the dominant bacteria were prepared according to a certain proportion, after purification and domestication, the bacteria were isolated and identified as 8 strains of bacteria with the ability of iron and manganese removal and ammonia nitrogen removal. According to the characteristics of groundwater quality in northern area (Shenyang), the groundwater quality was acclimated at low temperature, and the expected treatment effect was achieved under the environmental conditions. A one-month low temperature acclimation test was carried out on the dominant bacteria agent. After acclimation, the growth cycle was prolonged at 10 鈩，
本文编号：2304352
[Abstract]:Groundwater is an important source of water for domestic and industrial use. Since water pollution has not been effectively controlled, groundwater in some areas has been polluted to varying degrees, resulting in ammonia nitrogen and CODMn exceeding the standard, combined with primary iron and manganese, which form a complex micro-polluted groundwater, in which the removal of pollutants affects each other. It greatly increases the difficulty of groundwater treatment. Therefore, simultaneous removal of iron, manganese, ammonia nitrogen and CODMn, from groundwater has become an important issue in drinking water treatment. In this experiment, the low temperature acclimation of the new dominant microorganism was carried out to improve the oxidation effect by improving the slow growth and low bioactivity of the former microbes under the low temperature condition, and increasing the low temperature tolerance and the biological enzyme activity of the microorganism. In this paper, the technology of aeration and ceramsite adsorption is used to remove iron and manganese from groundwater. By inoculating the dominant bacteria at low temperature and strengthening the purifying function of filter, the purpose of treating compound micro-polluted groundwater effectively is achieved. The research contents include three parts: first, the low temperature acclimation test of dominant bacteria; second, the response surface method to determine the optimal growth conditions of dominant bacteria after acclimation; third, the field pilot test of treatment of groundwater with dominant bacteria after low temperature acclimation. After purification and domestication at room temperature, 5 strains of bacteria were selected for compound test, and the dominant bacteria were prepared according to a certain proportion, after purification and domestication, the bacteria were isolated and identified as 8 strains of bacteria with the ability of iron and manganese removal and ammonia nitrogen removal. According to the characteristics of groundwater quality in northern area (Shenyang), the groundwater quality was acclimated at low temperature, and the expected treatment effect was achieved under the environmental conditions. A one-month low temperature acclimation test was carried out on the dominant bacteria agent. After acclimation, the growth cycle was prolonged at 10 鈩，
本文编号：2304352
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