多重耐药铜绿假单胞菌的相关耐药机制研究

发布时间：2017-12-28 12:23

本文关键词：多重耐药铜绿假单胞菌的相关耐药机制研究　出处：《中国抗生素杂志》2016年05期 　论文类型：期刊论文

【摘要】：目的分析我院铜绿假单胞菌的耐药性与耐药机制,为临床抗感染治疗提供理论依据。方法采用K-B法进行体外药敏试验,收集耐碳青霉烯类铜绿假单胞菌60株,检测其主动外排泵表型,PCR扩增其外膜蛋白opr D2基因、金属酶相关基因,PFGE同源性分析等。结果我院耐碳青霉烯类耐药率较高,于2014年达8.3%。6株外排泵表型阳性,IMP阳性32份,VIM阳性16份,IMP和VIM均阳性12份,外膜蛋白Opr D2缺失3株。PFGE同源性分析,结果分为3个型。其中A型有25株,B型有20株,C型有15株。结论我院多重耐药铜绿假单胞菌检出率较高,耐药机制以IMP与VIM为主,同时存在外排泵及外膜蛋白耐药机制,PFGE同源性分析显示我院存在不同克隆株流行,需加强细菌爆发流行防护措施。
[Abstract]:Objective to analyze the drug resistance and resistance mechanism of Pseudomonas aeruginosa in our hospital, and to provide a theoretical basis for clinical anti infection treatment. Methods in vitro susceptibility test was carried out by K-B. 60 strains of carbapenem resistant Pseudomonas aeruginosa were collected. The active efflux pump phenotype was detected. The outer membrane protein OPR D2 gene, metalloenzyme related gene and PFGE homology analysis were amplified by PCR. Results the resistance rate of carbon penicenes was higher in our hospital, up to 8.3% in 2014. 6 strains of efflux pump were positive, IMP positive 32, VIM positive 16, both IMP and VIM positive 12, and outer membrane protein Opr D2 missing 3 strains. PFGE homology analysis, the results are divided into 3 types. Among them, there were 25 A, 20 B and 15 C. Conclusion the detection rate of multidrug-resistant Pseudomonas aeruginosa is high in our hospital. The mechanism of resistance is mainly IMP and VIM, and there is the mechanism of efflux pump and outer membrane protein resistance. PFGE homology analysis shows that there are different clones in our hospital. Therefore, it is necessary to strengthen the protection measures against bacterial outbreaks.
【作者单位】：瑞安市中医院内科;
【基金】：温州卫生局项目(No.2015B44) 瑞安市科技局(No.MS2015036)
【分类号】：R446.5
【正文快照】： 多重耐药铜绿假单胞菌的出现对临床治疗带来巨大的考验,近年来PA的耐药机制主要有以下几方面:主动外排系统的作用;抗生素水解酶和修饰酶的作用;菌体细胞膜通透性的改变等。我们通过收集临床2010—2014年间分离出来的铜绿假单胞菌,分析其耐药现状与耐药机制,对我院临床治疗与预

【相似文献】