输血相关急性肺损伤大鼠肺组织细胞因子诱导的中性粒细胞趋化因子的表达

发布时间：2017-12-31 12:32

本文关键词：输血相关急性肺损伤大鼠肺组织细胞因子诱导的中性粒细胞趋化因子的表达　出处：《中国呼吸与危重监护杂志》2016年04期 　论文类型：期刊论文

【摘要】：目的研究输血相关急性肺损伤(TRALI)大鼠肺组织细胞因子诱导的中性粒细胞趋化因子1(CINC-1)mRNA表达水平的变化,探讨CINC-1在大鼠TRALI发病机制中的作用。方法将60只SD大鼠随机分为TRALI组、正常对照组、脂多糖(LPS)对照组和阳性对照组,每组15只大鼠。LPS对照组大鼠经腹腔注射LPS(2 mg/kg,1 min内完毕),2 h后静脉输注1 m L生理盐水;TRALI组大鼠经腹腔注射LPS(2 mg/kg)2 h后静脉输注1 m L储存28 d的同种异体血浆;正常对照组大鼠采用假手术处理;阳性对照组大鼠移除约等于大鼠10%血容量的血液(1 m L)后,经静脉输注LPS(5 mg/kg)诱导急性肺损伤。采用逆转录聚合酶链反应检测大鼠肺组织中CINC-1 mRNA表达水平,采用酶联免疫吸附试验测定肺组织匀浆CINC-1蛋白含量;检测肺湿/干重比、肺组织髓过氧化物酶(MPO)活性,观察肺组织病理学改变,比较支气管肺泡灌洗液(BALF)中细胞总数和中性粒细胞百分比。结果与正常对照组和LPS对照组比较,TRALI组肺组织中CINC-1蛋白和CINC-1 mRNA表达明显增高[(18.98±2.21)ng/g和[(26.42±3.37)ng/g比(38.95±4.32)ng/g,F=11.28,P0.05;(0.24±0.09)和(0.15±0.08)比(0.38±0.09),F=15.31,P0.05]。TRALI组BALF中细胞总数和中性粒细胞百分比[(310.63±76.67)×106/L和(33.57±11.51)%]明显高于正常对照组[(101.36±63.83)×106/L和(9.87±3.56)%](F=13.82、5.41,P0.05)。TRALI组肺组织含水量和MPO活性明显高于正常对照组(F=5.78、13.46,P0.05)。阳性对照组各指标均显著高于TRALI组(P0.05)。结论 TRALI大鼠肺组织中CINC-1的表达升高,肺组织中性粒细胞浸润增多,提示CINC-1参与了中性粒细胞与内皮细胞的粘附和聚集,推测CINC-1可能在TRALI的发病过程中起着重要作用。
[Abstract]:Objective to study the blood transfusion related acute lung injury (TRALI) induced neutrophil cell factor in rat lung tissue chemokine 1 (CINC-1) expression of mRNA, to explore the role of CINC-1 in pathogenesis of TRALI in rats. Methods: 60 SD rats were randomly divided into TRALI group and normal control group. Lipopolysaccharide (LPS) control group and positive control group, 15 rats in each group.LPS control group rats by intraperitoneal injection of LPS (2 mg/kg, 1 min, 2 h.) after intravenous infusion of 1 m L saline; group TRALI rats by intraperitoneal injection of LPS (2 mg/kg) 2 h after intravenous infusion 1 m L 28 d storage allogeneic plasma; the rats in the normal control group with sham operation; positive control group rats 10% rats removed approximately equal to the blood volume of blood (1 m L) after intravenous infusion of LPS (5 mg/kg) induced acute lung injury. The expression of CINC-1 mRNA in lung tissue the rats were detected by reverse transcriptase polymerase chain reaction The level of determination of CINC-1 protein in lung tissue homogenate by enzyme-linked immunosorbent assay; detection of lung wet / dry weight ratio, lung tissue myeloperoxidase (MPO) activity, pathological changes of lung tissue, comparison of bronchoalveolar lavage fluid (BALF) in total cell and neutrophil percentage. Results compared with the normal control group and LPS compared to the control group, the lung tissue in TRALI group the expression of CINC-1 protein and CINC-1 mRNA significantly increased [(18.98 + 2.21) ng/g and [(26.42 + 3.37) than ng/g (38.95 + 4.32) ng/g, F=11.28, P0.05; (0.24 + 0.09) and (0.15 + 0.08) than (0.38 + 0.09), F=15.31. P0.05].TRALI BALF group in total cell and neutrophil percentage [(310.63 + 76.67) * 106/L and (33.57 + 11.51) when it was significantly higher than normal control group [(101.36 + 63.83) * 106/L and (9.87 + 3.56) when (F=13.82,5.41, P0.05).TRALI group of lung tissue water content and the MPO activity was significantly higher than that of normal control group (F=5.78,1 3.46, P0.05). The index of positive control group were significantly higher than that of TRALI group (P0.05). The increased expression of CINC-1 in rat lung tissue conclusion in TRALI lung tissue, increased neutrophil infiltration, suggesting that CINC-1 is involved in the adhesion of neutrophils to endothelial cells and aggregation, suggesting that CINC-1 may play an important role in the pathogenesis of in the process of TRALI.

【作者单位】：郑州大学第五附属医院输血科;
【分类号】：R457.1;R563
【正文快照】： 随着血液采集、血制品制备过程中消毒灭菌技术的提高和操作规程的不断完善,多数输血相关并发症已得到了较满意的控制[1]。但免疫相关性输血并发症问题日益突出[2],输血相关急性肺损伤(transfusion-related acute lung injury,TRALI)就是其中之一。它通常发生于输血期间或输血

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