点免疫金渗滤法筛选胶体金标记抗人IgG的研究

发布时间：2018-01-01 22:32

本文关键词：点免疫金渗滤法筛选胶体金标记抗人IgG的研究　出处：《中国免疫学杂志》2016年12期 　论文类型：期刊论文

【摘要】：目的:应用点免疫金渗滤法筛选适宜胶体金标记的抗人IgG(以下简称二抗),并运用多个评价指标进行比较和分析。方法:选择三个不同公司的二抗用胶体金标记,根据棋盘滴定法确定最佳标记条件;运用点免疫金渗滤法对三种二抗的胶体金标记后检测效能进行比较,采用包虫病人和健康对照血清,用包虫病特异性抗原检测包虫病患者血清中的特异性抗体,评价最优的二抗,并与酶联免疫吸附测定法进行比较。结果:A、B、C三种二抗标记的最佳标记条件为:p H均为8.5,加入量分别为38.4、24、19.2μg/ml,综合评价二抗B检测效能最优。将其与酶联免疫吸附测定法检测效能进行比较,两种方法检测结果的Kappa=0.895(P0.05),吻合度较强。结论:应用点免疫金渗滤法,以及本文提出的评价指标,能够筛选出最佳二抗,对检测试剂盒中二抗的选择具有重要的参考价值。
[Abstract]:Objective: the application of dot immunogold filtration assay for screening of colloidal gold labeled anti human IgG (hereinafter referred to as the two anti), and the use of multiple evaluation indexes were compared and analyzed. Methods: three different companies with two anti colloidal gold, according to determine the optimal labeling conditions with chessboard titration; immune gold filtration assay to compare three kinds of colloidal gold labeled anti two note after detection efficiency, the patients with hydatid disease and healthy control serum, with specific antibody of echinococcosis specific antigen in sera of patients with hydatid disease detection, evaluation of optimal two antibody, and ELISA method were compared. Results: A B, C, the best labeling conditions three two anti markers: P H = 8.5, adding 38.4,24,19.2 g/ml respectively, two comprehensive evaluation of anti B detection efficiency. The optimal ELISA detection efficiency comparison, the test results of the two methods Kappa=0.895 (P0.05) has strong agreement. Conclusion: the application of dot immunogold filtration assay and the evaluation index proposed in this paper can screen the best two resistance, which has important reference value for the selection of the two antibody in the detection kit.

【作者单位】：新疆医科大学第一附属医院临床医学研究院;
【基金】：新疆医科大学第一附属医院科研奖励基金(2011YFY16)
【分类号】：R446.6
【正文快照】： 1本文为新疆医科大学第一附属医院科研奖励基金(2011YFY16)。2并列第一作者。胶体金作为快速简便的示踪剂,近年来在疾病的快速检测和床边检测显示出其优势,尤其是在临床免疫学检测中,如早早孕试纸条、艾滋病快速检测卡等。我单位研制并成功注册的包虫病特异性抗体检测试剂盒(

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