聚肌苷酸胞苷酸增强间充质干细胞对脓毒症大鼠肠屏障功能的保护作用

发布时间：2018-01-02 11:53

本文关键词：聚肌苷酸胞苷酸增强间充质干细胞对脓毒症大鼠肠屏障功能的保护作用　出处：《第三军医大学学报》2016年09期 　论文类型：期刊论文

【摘要】：目的观察间充质干细胞(mesenchymal stem cells,MSCs)及聚肌苷酸胞苷酸poly(I:C)预刺激MSCs对脓毒症大鼠肠屏障功能的保护作用。方法采用盲肠结扎穿孔(cecal ligation puncture,CLP)诱导大鼠脓毒症模型,将SD大鼠随机分为5个组:正常对照组、脓毒症组、常规治疗组(乳酸林格液体复苏+血管活性药多巴胺+头孢呋辛钠)、MSCs治疗组(MSCs 3×106/只+常规治疗)和ploy(I:C)预刺激MSCs组[poly(I:C)20μg/m L预刺激MSCs 24 h+常规治疗],观察不同处理组大鼠肠通透性,血中D乳酸、细菌内毒素浓度、肠病理组织形态及动物存活时间、存活率的变化。结果与正常对照组相比,脓毒症大鼠肠通透性、血中D乳酸、细菌内毒素浓度显著增高(P0.01),病理结果显示肠绒毛断裂、减少,上皮细胞坏死脱落,炎细胞、红细胞增多。常规治疗后第3天,同脓毒症组相比,大鼠肠通透性有改善,血D乳酸、细菌内毒素含量降低,肠绒毛损害及炎症减轻,长度增加;MSCs+常规治疗后效果明显优于常规治疗组,而poly(I:C)预刺激MSC可增强MSCs对肠屏障功能的保护作用,肠通透性及血中D乳酸、内毒素浓度显著降低(P0.05),病理结果显示肠上皮增生修复,肠绒毛增多,炎症明显减轻。结论 poly(I:C)可增强MSC对脓毒症大鼠肠屏障功能的保护作用。
[Abstract]:Objective To observe the effect of mesenchymal stem cells (mesenchymal stem cells, MSCs) and polyinosinic polycytidylic acid poly (I:C) preconditioning protective effect of MSCs on sepsis with intestinal barrier function of rats. Methods by cecal ligation and puncture (cecal, ligation puncture, CLP) induced septic rat model, SD rats were randomly divided into 5 groups: normal control group, sepsis group, routine treatment group (lactated Ringer's fluid resuscitation + vasoactive drugs, dopamine and cefuroxime sodium) MSCs treatment group (MSCs 3 * 106/ + routine treatment) and Ploy (I:C) - stimulated MSCs group (I:C) [poly 20 g/m L MSCs 24 h+ pre stimulation treatment, observation of different treatment groups of rat intestinal permeability, blood lactic acid D, bacterial endotoxin concentration, intestinal histopathological and animal survival time, the change in survival rate. Results compared with the normal control group, sepsis rat intestinal permeability, blood lactic acid D, bacterial endotoxin concentration a significant increase High (P0.01), pathological results showed intestinal villi fracture reduction, epithelial cell necrosis, inflammatory cells, red blood cells increased. Third days after routine treatment, compared with the sepsis group, rat intestinal permeability improved, blood lactic acid D, reduce the content of bacterial endotoxin, intestinal villus injury and inflammation, increase the length of; the effect of the treatment group was significantly better than the conventional MSCs+ after routine treatment, while poly (I:C) pre stimulation MSC can enhance the protective effect of MSCs on intestinal barrier function, intestinal permeability and blood lactic acid in D, endotoxin concentrations were significantly decreased (P0.05), pathological results showed hyperplasia of intestinal epithelial repair, intestinal villi increased significantly reduced inflammation. Conclusion poly (I:C) can enhance the protective effect of MSC on sepsis with intestinal barrier function of rats.

【作者单位】：第三军医大学大坪医院野战外科研究所第二研究室创伤、烧伤与复合伤国家重点实验室;
【基金】：国家自然科学基金面上项目(81570441)~~
【分类号】：R459.7
【正文快照】： 创伤、烧伤与复合伤国家重点实验室)Supported by the General Program of National Natural Science Foundation of China(81570441).Corresponding author:Liu Liangming,E-mail:liangmingliu@yahoo.com脓毒症是临床常见的危重病症。据统计全球每年有2 100万脓毒症患者,其发

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