微柱凝胶卡漏检B_w03亚型分析——附2例报告

发布时间：2018-01-02 20:30

本文关键词：微柱凝胶卡漏检B_w03亚型分析——附2例报告　出处：《中国输血杂志》2016年10期 　论文类型：期刊论文

【摘要】：目的对2例微柱凝胶卡漏检B_w03亚型标本血型血清学特点及其分子遗传背景进行系统分析,以期为临床安全输血提供帮助。方法分别应用试管法和微柱凝胶卡法进行血型正反定型;应用PCR-SSP方法对ABO血型基因进行初步分型;并进一步应用直接测序和单体型序列分析法对ABO基因第6、7外显子进行分析以确定其基因型。结果标本1:试管法结果疑似B亚型,凝胶卡结果正定为O型反定为B型,基因检测结果为B_w03。标本2:试管法结果疑似AB亚型,凝胶卡结果正定为A型反定为AB型,基因检测结果为AB_w03。结论微柱凝胶卡能漏检B_w03亚型,应采用多种方法进行血型鉴定以减少临床血型鉴定错误及相关输血反应的发生。
[Abstract]:Objective to systematically analyze the blood group serological characteristics and molecular genetic background of two microcolumn gel card missed Bw03 subtype specimens. Methods Blood group positive and negative typing were performed by using test tube method and microcolumn gel card method respectively. The genotyping of ABO blood group gene was carried out by PCR-SSP method. Furthermore, direct sequencing and haplotype sequence analysis were used to analyze exon 6 of ABO gene to determine its genotype. The results of gel card were positively determined as O type and B type, and the result of gene detection was B / W 03. Specimen 2: the test tube method showed that AB subtype was suspected, and the positive definite result of gel card was A type instead of AB type. Conclusion Microcolumn gel card can not detect Bw03 subtype, and blood type identification should be carried out by various methods to reduce the occurrence of clinical blood group identification error and related blood transfusion reaction.
【作者单位】：青岛市中心血站输血医学研究所;青岛市海慈医疗集团检验科;
【基金】：青岛市市南区科技发展资金项目(2013-13-013-YY) 青岛市医疗卫生优秀人才培养项目
【分类号】：R457.1
【正文快照】： quence analysis of exons 6 and 7 of ABO gene was performed by sequence-based testing and cloning sequencing.ResultsThe result of sample 1 from tube test was possibly B subtype.The results of positive determination was O and negative deter-mination was B.

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