西藏芽孢杆菌XZ7中Amicoumacin类抗生素的分离鉴定与生物活性研究

发布时间：2018-01-10 08:32

本文关键词：西藏芽孢杆菌XZ7中Amicoumacin类抗生素的分离鉴定与生物活性研究　出处：《天然产物研究与开发》2016年01期 　论文类型：期刊论文

【摘要】：采用UPLC-DAD-MS分析发现西藏当雄土壤来源芽孢杆菌XZ7发酵液中的Amicoumacin类化合物,并利用反相柱层析和高效液相色谱对发现的3个Amicoumacin类化合物进行分离纯化;根据紫外光谱、高分辨质谱和核磁共振波谱数据及文献比对,鉴定3个化合物分别为:Bacilosarcin A(1)、Bacilosarcin B(2)和Bacilosarcin C(3)。抑菌实验结果表明化合物2对表皮葡萄球菌和金黄色葡萄球菌具有较强的抑制活性(MIC=4~16μg/m L);细胞毒性测试结果表明化合物1和2对HepG2肿瘤细胞具有较高的抑制作用,其中化合物2抑制作用最强,IC_(50)值为2.8μM。同时,化合物2对MCF-7和He La肿瘤细胞也有较强的细胞毒作用,IC_(50)值分别为6.2μM和4.0μM。
[Abstract]:UPLC-DAD-MS analysis was used to identify Amicoumacin compounds in XZ7 fermentation broth of Bacillus sp. from Dangxiong soil in Tibet. Three Amicoumacin compounds were separated and purified by reversed-phase column chromatography and high performance liquid chromatography. According to the UV spectrum, high resolution mass spectrometry and nuclear magnetic resonance spectroscopy data and literature comparison, three compounds were identified as: 1 Bacilosarcin AH-1). Bacilosarcin Bn2) and Bacilosarcin Cf3). The results of bacteriostatic test showed that compound 2 had strong inhibitory activity on Staphylococcus epidermidis and Staphylococcus aureus. The results of cytotoxicity test showed that compounds 1 and 2 had a high inhibitory effect on HepG2 tumor cells, and compound 2 had the strongest inhibitory effect on HepG2 tumor cells. The inhibitory value of compound 2 was 2.8 渭 M. at the same time. Compound 2 also had a strong cytotoxic effect on MCF-7 and He-La tumor cells. The IC50 values were 6.2 渭 M and 4.0 渭 M, respectively.
【作者单位】：中国医学科学院&北京协和医学院医药生物技术研究所;中国药科大学生命科学与技术学院;
【基金】：国家自然科学基金(81402834和81172963) 北京市自然科学基金(7154223)
【分类号】：R446.5
【正文快照】： Amicoumacin类抗生素是一类具有8-羟基-3,4-二氢异香豆素母核结构(如图1所示)的抗生素,自1975年第一个明确报道结构的化合物Baciphelac-in[1]发现至今,天然来源的此类化合物至少已发现36个,产生菌绝大多数是芽孢杆菌。由于携带的羟基化酰氨基侧链不同,使该类化合物结构多样并

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