临床分离的Janibacter全基因组从头测序及比较基因组学研究

发布时间：2018-01-11 00:23

本文关键词：临床分离的Janibacter全基因组从头测序及比较基因组学研究　出处：《南昌大学》2015年硕士论文　论文类型：学位论文

【摘要】：本论文在临床微生物诊断中,发现并分离了一株少见细菌—Janibacter。我们首先通过扩增该分离株的16s rDNA并进行测序、序列比对,确定该分离株属于Janibacter菌属,因此,命名为Janibacter YFY001株。与此同时,我们验证了该细菌的药敏实验,发现JanibacterYFY001株对利福平、头孢他啶和克林霉素耐药。由于该菌属于高GC%含量菌种,所以,我们结合第二代和第三代基因组测序技术,对所提取的Janibacter全基因组进行测序,并获得了YFY001株的全基因组信息。YFY001株全基因组大小为3.40Mbp,GC%为71.46%。随后,我们对其中可能与致病相关的调控系统以及致病因子,进行注释;并借助比较基因组的研究方法,将YFY001株与已经报道的两株环境株:HTCC2469和holey进行比较分析。最终发现了911个核心蛋白;而有1039个蛋白特异地存在于YFY001株中。本论文借助全基因组测序的方法,对临床分离的高GC含量的Janibacter YFY001,结合了第二代和第三代测序技术,最终获得了YFY001株的全基因组序列。在YFY001株的全基因组中,我们发现了双组份调控系统、分泌系统等与致病性相关的蛋白。与环境株的比较基因组发现特异存在于YFY001株的蛋白可能也与其致病性有一定的关系。本研究结合基因组测序技术,对临床分离的少见菌株进行全基因组测序,对于阐明这类细菌的致病因子,为感染病人提供及时的治疗提供必要的依据。
[Abstract]:This paper in clinical microbiology diagnosis, discovered and isolated a rare bacterial strain Janibacter. we first amplified by the isolates 16S and rDNA sequencing, sequence alignment, the isolates belonging to Janibacter genus, therefore, named as Janibacter YFY001 strain. At the same time, we tested the bacterial susceptibility test the discovery of JanibacterYFY001 strain of Li Fuping, ceftazidime and clindamycin resistance. Due to the high content of bacteria belonging to GC% strains, so we combine the second and third generation genome sequencing, sequencing of Janibacter extracted from whole genome, and obtained the whole genome.YFY001 YFY001 strain genome size is 3.40Mbp. GC% 71.46%. then, we make comments on the regulation system which may be related to virulence and pathogenic factor; and by means of comparative genomics method, YFY001 Strains and two strains of environmental strains have been reported: HTCC2469 and holey were compared and analyzed. Finally found 911 core protein; and 1039 proteins specific to YFY001 strain. This paper using whole genome sequencing, high content of GC in clinical isolates of Janibacter YFY001, with second and third generation of sequencing technology, finally got the whole genome sequence of YFY001 strain. In the genome of YFY001 strain in, we found two component regulatory system, proteins related to the pathogenicity of the secretion system. Compared with the genome environment strain found in specific strain YFY001 protein may also have a certain relationship with pathogenicity this study combined with DNA sequencing technology, whole genome sequencing of rare clinical strains, to clarify the pathogenic factors of bacteria, to provide the necessary basis for the patients to provide timely treatment According to.

【学位授予单位】：南昌大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R446.5

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