转化生长因子-β1和血管内皮生长因子在浓缩生长因子各层中的分布及含量特点

发布时间：2018-01-12 18:34

本文关键词：转化生长因子-β1和血管内皮生长因子在浓缩生长因子各层中的分布及含量特点　出处：《北京大学学报(医学版)》2016年05期 　论文类型：期刊论文

【摘要】：目的:测定浓缩生长因子(concentrated growth factors,CGF)凝胶层与红细胞(red blood cells,RBC)层中转化生长因子-β1(transforming growth factor-β1,TGF-β1)、血管内皮生长因子(vascular endothelial growth factor,VEGF)分布及含量,探究TGF-β1、VEGF在CGF凝胶层内的分布及含量与其促成骨效应的相关性并指导临床应用。方法:招募6名健康志愿者,采集静脉血制备CGF样品。免疫组织化学法观察CGF凝胶层与RBC层中TGF-β1、VEGF的分布并测定制备即刻的累积光密度值(integrated optical density,IOD)和平均光密度值(average optical density,AOD)。酶联免疫吸附(enzyme-linked immunosorbent assays,ELISA)测定制备即刻上清层与制备1 d后CGF凝胶层析出液中TGF-β1、VEGF的浓度。结果:TGF-β1与VEGF大量聚集于CGF凝胶层中,在RBC层中仅位于多核细胞胞浆和散在的血小板内;CGF凝胶层与RBC层的交界区域有大量血小板与白细胞聚集,并有较高的TGF-β1表达;CGF凝胶层析出液中TGF-β1和VEGF的浓度分别为(55 236.78±3 686.34)ng/L和(610.99±148.81)ng/L,均显著高于上清层(20 710.20±4 523.14)ng/L和(335.20±51.69)ng/L(P0.001)。结论:CGF凝胶层内含有大量TGF-β1、VEGF,可发挥促进成骨与加速组织愈合的作用。在临床应用中建议应用时间宜早为佳,除整个CGF层外,可将上清层、CGF层与RBC层交界区域一并使用。
[Abstract]:Objective: to determine the gel layer of concentrated growth factor concented growth factors (CGF) and red blood cells in erythrocytes. Transforming growth factor- 尾 1 (TGF- 尾 1). The distribution and content of vascular endothelial growth factor (VEGF) in vascular endothelial growth factor.TGF- 尾 1 was studied. The relationship between the distribution and content of VEGF in CGF gel layer and its effect on bone and guiding clinical application. Methods: 6 healthy volunteers were recruited. CGF samples were collected from venous blood, and TGF- 尾 1 in CGF gel layer and RBC layer were observed by immunohistochemical method. The distribution of VEGF and the immediate cumulative optical density of the prepared optical density were measured. IOD) and average optical density. Enzyme linked immunosorbent assays. TGF- 尾 1 in CGF gel chromatography solution was determined by Elisa. The concentration of VEGF. Results: TGF- 尾 1 and VEGF were clustered in the CGF gel layer, and in the RBC layer only in the cytoplasm of the multicellular cells and in the scattered platelets. A large number of platelets and leukocytes were accumulated in the junction region of CGF gel layer and RBC layer, and the expression of TGF- 尾 1 was higher. The concentrations of TGF- 尾 1 and VEGF in CGF gel chromatography solution were 55 236.78 卤3 686.34 ng / L and 610.99 卤148.81, respectively. Ng/L. Both were significantly higher than those in the upper clear layer (20 710.20 卤4 523.14 ng / L and 335.20 卤51.69 ng / L P 0.001). Conclusion: TGF- 尾 1 was found in the gel layer of CGF. VEGF, which can promote osteogenesis and accelerate tissue healing, should be applied early in clinical application, except for the whole CGF layer, the supernatant layer can be added. The CGF layer is used in conjunction with the RBC layer.
【作者单位】：北京大学口腔医学院·口腔医院修复科口腔数字化医疗技术和材料国家工程实验室口腔数字医学北京市重点实验室;中国人民解放军总医院口腔科;
【基金】：国家临床重点专科建设项目(2011) 教育部留学回国人员科研启动基金(2012-940)资助~~
【分类号】：R446.6
【正文快照】： 浓缩生长因子(concentrated growth factors,CGF)是继富血小板血浆(platelet-rich plasma,PRP)、富血小板纤维蛋白(platelet-rich fibrin,PRF)后的新一代血小板浓缩产物,由Saccol于2006年首先提出[1],其包含了发挥骨诱导作用的自体血小板生长因子和发挥骨引导作用的纤维蛋白基

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