血清学联合核酸检测在献血者血液筛查中的互补性

发布时间：2018-01-19 09:47

  本文关键词： 核酸检测 血清学检测 血液筛查　出处：《中国艾滋病性病》2016年03期 　论文类型：期刊论文

【摘要】：目的对温州地区无偿献血者的血液标本进行血清学与核酸联合检测,全面评估血清学与核酸检测(NAT)在降低输血相关传染性疾病中的互补性。方法对2014年10月至2015年6月,温州市中心血站采集的无偿献血者的血液标本,用酶联免疫吸附试验(ELISA)对血液传染性指标乙型肝炎病毒表面抗原(HBsAg)、丙型肝炎病毒(HCV)抗体、艾滋病病毒(HIV)抗体、苍白球螺旋体(TP)抗体进行检测,丙氨酸转氨酶(ALT)采用速率法,同时采用核酸检测技术对乙型肝炎病毒(HBV)DNA、HCV RNA、HIV RNA进行6人份三项目联合检测,并对检测结果进行分析。结果 33 736份血液标本中,ELISA(+)NAT(+)95份,其中HBsAg(+)NAT(+)72份,符合率60.5%;HCV抗体(+)NAT(+)13份,符合率11.2%;HIV抗体(+)NAT(+)10份,符合率30.3%。41份ELISA(-)核酸(+)样本,且均为HBV DNA阳性。ELISA(+)NAT(-)173份。ELISA双试剂阳性样本中,HBV、HCV、HIV S/CO≥5的样本组的核酸阳性符合率高于S/CO5的样本组。结论核酸检测能有效地降低输血传播疾病的发生,但也存在漏检的可能,不能完全取代血清学检测,只有两者有机结合才能真正保证血液的安全。
[Abstract]:Objective to detect the serum and nucleic acid of blood samples from unpaid blood donors in Wenzhou area. A comprehensive assessment of the complementarity between serology and nucleic acid testing (Nat) in reducing transfusion-related infectious diseases was conducted from October 2014 to June 2015. Blood samples collected from blood donors from Wenzhou city center blood station were tested by Elisa to detect hepatitis B virus surface antigen (HBsAg). The antibody against hepatitis C virus (HCV), HIV / AIDS (HIV / AIDS) and Treponema pallidum (TPTP) were detected, and alanine aminotransferase (alt) was used as rate method. At the same time, the nucleic acid detection technique was used to detect the RNA of hepatitis B virus (HBV) in hepatitis B virus (HBV) DNA. Results of 33 736 blood samples, 95 were Nat (Nat) and 72 were HBsAg (Nat). The coincidence rate was 60.5%; HCV antibody (Nat 13 copies, coincidence rate 11.2%); HIV antibody (Nat 10, coincidence rate 30.3.41 ELISA-) nucleic acid () samples. All the samples were positive for HBV DNA. The positive coincidence rate of nucleic acid in the HIV / CO 鈮，

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