评估一种新的检测瘰疬分枝杆菌菌株的real-time PCR方法（英文）

发布时间：2018-01-21 21:11

  本文关键词： 瘰疬分枝杆菌 real-time PCR 非结核分枝杆菌　出处：《中国人兽共患病学报》2016年12期 　论文类型：期刊论文

【摘要】：目的建立针对非结核分枝杆菌中瘰疬分枝杆菌菌株的real-time PCR检测方法。方法在我们的研究中,根据瘰疬分枝杆菌的SodA基因设计MGB探针和特定的引物,并用来检测模拟样品中的瘰疬分枝杆菌。结果该方法最低瘰疬分枝杆菌DNA检测浓度是101拷贝数,标准曲线显示阈值周期和SodA基因片段拷贝数之间的相关系数是0.973,斜率为-3.249,表现出良好的线性关系。此外,模拟样品最低检测浓度是每毫升101个细菌,此外,其他9株菌为阴性结果,验证了良好的特异性。结论该方法对于检测瘰疬分枝杆菌模拟标本显示很高的敏感性和特异性,可用于瘰疬分枝杆菌菌株的生态和流行病学监测。
[Abstract]:Objective to establish a real-time PCR method for the detection of Mycobacterium scrofulla in non-tuberculous mycobacteria. MGB probes and specific primers were designed according to the SodA gene of Mycobacterium scrofulla. The method was used to detect Mycobacterium scrofula in simulated samples. Results the lowest DNA detection concentration of Mycobacterium scrofula was 101-copy number. The standard curve showed that the correlation coefficient between threshold period and copy number of SodA gene fragment was 0.973, slope was -3.249, showing a good linear relationship. The minimum detectable concentration of simulated samples was 101 bacteria per milliliter, in addition, the other 9 strains were negative. Conclusion this method is highly sensitive and specific for the detection of mimic specimens of Mycobacterium scrofula, and can be used for ecological and epidemiological surveillance of Mycobacterium scrofula.
【作者单位】： 中国疾病预防控制中心传染病预防控制所 传染病预防控制国家重点实验室 感染性疾病诊治协同创新中心;温州医科大学检验医学院生命科学学院;
【基金】：financially supported by the projects 81401647 of Natural Science Foundation of China 2013ZX10003006 and 2013ZX10003002001 of Chinese National Key Program of Mega Infectious Diseases~~
【分类号】：R440
【正文快照】： IntroductionM.scrofulaceumis　a　slow-growing　environ-Mycobacteria　that　are　not　members　of　the　M.mental　and　opportunistic　atypical　mycobacteriumtuberculosis　complex(M.africanum,M.bovis,that　causes　cervical　lymphadenopathy　in　childrenM.b，

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