丙酮酸钠改善红细胞储存损伤的初步探讨

发布时间：2018-01-21 22:41

  本文关键词： 丙酮酸钠 储存红细胞 储存损伤 氧化损伤 能量供应 动物实验 小鼠　出处：《中国输血杂志》2016年04期 　论文类型：期刊论文

【摘要】：目的探讨丙酮酸钠(SP)对红细胞储存损伤的影响。方法戊巴比妥钠腹腔注射麻醉小鼠,同时机械通气,通过心脏穿刺取血,CPDA-1作为抗凝剂和储存液,过滤去除白细胞后平分2组(n=8),SP组:添加SP浓缩液使储存红细胞中SP的终浓度为2.5 mmol/L;生理盐水(NS)组:按相同体积比加入等量的生理盐水。400 g离心15min,调整Hct为70%-75%,4℃储存14 d后,用全自动血细胞计数仪测定储存红细胞的血常规;分别用三磷酸腺苷(ATP)、丙二醛(MDA)测定试剂盒测定储存红细胞中ATP、MDA水平。结果储存14 d时,2组血细胞计数各指标无明显差异(P0.05),且均在正常范围。NS与SP组红细胞中ATP含量(nmol/mg)为:2.46±0.19 vs 2.82±0.21(P0.05);MDA含量(nmol/mg)为15.45±0.33 vs 14.66±0.87(P0.05)。结论在红细胞储存过程中,SP明显提高了储存红细胞ATP水平、降低了MDA水平,具有改善红细胞储存损伤的作用。
[Abstract]:Objective to investigate the effect of sodium pyruvate (SPN) on erythrocyte storage injury. Methods pentobarbital sodium was injected intraperitoneally to anesthetized mice and mechanical ventilation was used to collect blood by heart puncture. CPDA-1 was used as anticoagulant and storage liquid. After leucocyte removal, CPDA-1 was divided into two groups: the final concentration of SP in stored red blood cells was 2.5 mmol / L by adding SP concentrate. NS group: the rats were centrifuged with the same volume ratio of normal saline. 400g for 15 min, and the Hct was adjusted to 70 -75 鈩，

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