2012-2015年某传染病医院血流感染细菌构成及耐药性分析

发布时间：2018-02-12 08:35

本文关键词： 血流感染血培养病原菌构成耐药分析　出处：《现代预防医学》2016年15期 　论文类型：期刊论文

【摘要】：目的了解全院血流感染分离菌的临床分布及耐药性,为临床诊断及治疗提供依据。方法采用Bac T/Alert 3D240全自动血培养仪对全院2012年至2015年收集的9 923份血液标本进行培养,采用VITEK2 COMPACT 60对分离的细菌进行鉴定和药敏试验。结果 9 923份血标本中,656份检出微生物,阳性率6.6%。共分离出658株病原菌,其中革兰阳性菌占41.2%;革兰阴性菌占37.1%;真菌占21.7%。病原菌种类主要为凝固酶阴性葡萄球菌(24.2%)、新型隐球菌(14.9%)、大肠埃希菌(13.5%)、沙门菌属(6.7%)、马尔尼菲青霉菌(5.8%)。分离的大肠埃希菌和肺炎克雷伯菌对氨苄西林的耐药率分别为88.8%和100.0%,两者ESBLS的检出率分别为52.8%和11.1%;而凝固酶阴性葡萄球菌和金黄色葡萄球菌对青霉素的耐药率最高,分别为96.6%和100.0%,二者头孢西丁筛查阳性率分别为85.8%和13.6%。结论全院血流感染病原菌以凝固酶阴性葡萄球菌为主,新型隐球菌、大肠埃希菌和沙门菌属的检出率也较高,应予以高度重视。
[Abstract]:Objective to investigate the clinical distribution and drug resistance of blood stream infection isolates in our hospital, and to provide evidence for clinical diagnosis and treatment. Methods Bac T / Alert 3D240 automatic blood culture instrument was used to culture 9 923 blood samples collected from 2012 to 2015 in our hospital. VITEK2 COMPACT 60 was used to identify the isolated bacteria and drug sensitivity test. Results 656 microbes were detected in 9 923 blood samples, and the positive rate was 6.6. 658 strains of pathogenic bacteria were isolated. Gram-positive bacteria accounted for 41.2%; Gram-negative bacteria 37.1%; fungi 21.7.The main pathogens were coagulase negative staphylococci (24.22cm), Cryptococcus neoformans 14.9m, Escherichia coli 13.5m, Salmonella 6.7m, Penicillium marneffei 5.80.The isolated Escherichia coli was isolated. The resistance rates of Klebsiella pneumoniae and Klebsiella pneumoniae to ampicillin were 88.8% and 100.00.The detection rate of ESBLS was 52.8% and 11.1, respectively, while coagulase-negative staphylococci and Staphylococcus aureus had the highest resistance to penicillin. The positive rates of cefoxitin screening were 85.8% and 13.60.Conclusion coagulase negative staphylococcus was the main pathogen of blood stream infection in our hospital, and Cryptococcus neoformans, Escherichia coli and Salmonella were also detected. We should attach great importance to it.
【作者单位】：四川大学华西公共卫生学院(华西第四医院);成都市公共卫生临床医疗中心检验科;
【基金】：基金项目:省卫生厅090015
【分类号】：R446.5

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