丝网印刷电极的制备及其在内源性物质检测中应用

发布时间：2018-02-21 08:45

本文关键词： 雌二醇酪氨酸丝网印刷电极　出处：《湖北科技学院》2015年硕士论文　论文类型：学位论文

【摘要】：目的:制备出可以进行快速、准确测定的丝网印刷电极(Screen printed electrodes,SPE),将制备好的丝网印刷电极进行修饰,在合适的条件下用于雌二醇(Estradiol,E2)、酪氨酸(Tyrosine,Tyr)的测定。方法:采用丝网印刷技术制备丝印传感器,通过滴涂多壁碳纳米管(Mμlti-walled carbon nanotubes,MWNT)十六烷基三甲基溴化铵(Hexadecyl trimethyl ammonium bromide,CTAB)修饰液,提高电极灵敏度,并对制备条件和测定条件进行优化,制备出灵敏度高、重现性好、稳定性强的传感器。将制备好的即抛型电极(MWNT/CTAB-SPE),用于雌二醇、酪氨酸的测定。结果:本文采用丝网印刷技术制备出三电极测定体系,通过对制备条件和测定条件的筛选,用0.5 mg/mL 2μL的MWNT/CTAB复合修饰液用于修饰工作电极,选用0.1 M的pH 12.0的Na2CO3/硼砂缓冲液作为反应缓冲体系,用方波伏安法扫描的雌二醇的氧化峰电流与5×10-11~1×10-8 mol/L范围的浓度呈现很好的线性关系,其线性回归方程为:Ip(μA)=2.147C(10-8 mol/L)+0.043,R=0.9968。最低检测限为0.05 nmol/L。将该电极用于血样中雌二醇的含量测定,检测结果令人满意。选用1M硫酸作为电解质体系,用示差脉冲伏安法测定酪氨酸,酪氨酸在3.0×10-6~2.0×10-3 mol/L浓度范围,峰电流与浓度呈良好的线性关系,回归方程为Ip(μA)=0.048C(μmol/L)+0.315,R=0.9994。最低检测限为2.9×10-7mol/L。结论:本文研制出的即抛型传感器(MWNT/CTAB-SPE)灵敏度高,重现性好,能进行批量印制,便于标准化生产。该电极能够测定雌二醇、酪氨酸,满足快速便捷的诊断目的,有可能成为临床上用于检测的一种潜在检测手段而具有广大应用前景。
[Abstract]:Objective: to prepare a screen printing electrode, which can be measured quickly and accurately, and modify the prepared screen printing electrode. Methods: screen printing sensor was prepared by silk-screen printing technique. The modified solution of Hexadecyl trimethyl trimethyl ammonium bromideCTAB was prepared by using multi-walled carbon nanotubes M 渭 lti-walled carbon nanotubesMWNTs, and was used for the determination of estradiol Estradiolium E2O, tyrosine tyrosine tyrosine under suitable conditions. The sensor with high sensitivity, good reproducibility and stability was prepared by improving the electrode sensitivity and optimizing the preparation and determination conditions. The MWNT / CTAB-SPEN electrode was prepared and used for estradiol. Results: the three-electrode system was prepared by screen printing technique. By screening the preparation and determination conditions, the working electrode was modified with 0.5 mg/mL 2 渭 L MWNT/CTAB compound modification solution. The oxidation peak current of estradiol scanned by square wave voltammetry showed a good linear relationship with the concentration of 5 脳 10 ~ (-11) N _ (-1) 脳 10 ~ (-8) mol/L, using Na _ 2CO _ 3 / borax buffer solution of 0.1m pH 12.0 as the reaction buffer system, and the oxidation peak current of estradiol scanned by square wave voltammetry showed a good linear relationship. The linear regression equation is: 1: Ip (渭 Ip 2.147CU 10-8 mol / L) 0.043 mol / L, the lowest detection limit is 0.05 nmol / L. the electrode has been applied to the determination of estradiol in blood samples with satisfactory results. 1m sulfuric acid was used as electrolyte system and tyrosine was determined by differential pulse voltammetry. The linear relationship between the peak current and the concentration of tyrosine in the concentration range of 3.0 脳 10 ~ (-6) C ~ (-2) 脳 10 ~ (-3) is good. The regression equation is I _ p (渭 A ~ (-1 / L)) 0.315C (渭 mol / L) 0.315mol / L ~ (0.9994). The lowest detection limit is 2.9 脳 10 ~ (-7) mol / L 路L ~ (-1). Conclusion: the developed projectile sensor MWNT-CTAB-SPE has high sensitivity, good reproducibility and can be printed in bulk. The electrode can be used for the determination of estradiol and tyrosine, which can meet the rapid and convenient diagnostic purposes. It may become a potential detection method for clinical detection and has a broad application prospect.
【学位授予单位】：湖北科技学院
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R446;TP212

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