n-3多不饱和脂肪酸抑制SphK1信号通路改善脓毒症的炎性反应

发布时间：2018-02-21 21:12

  本文关键词： 脓毒症 n-多不饱和脂肪酸 鞘氨醇激酶 磷酸鞘氨醇受体　出处：《肠外与肠内营养》2016年05期 　论文类型：期刊论文

【摘要】：目的:观察富含n-3多不饱和脂肪酸(n-3 PUFA)的肠外营养(PN)能否通过鞘氨醇激酶1(Sph K1)和磷酸鞘氨醇受体(S1P)途径影响机体的炎性反应。方法:对大鼠实施颈外静脉置管,2 d后行盲肠结扎穿孔,然后随机分为等渗盐水组(静脉输注等渗盐水,n=20)、鱼油组(鱼油+PN,n=20)和大豆油组(PN,n=20),随后行PN支持5 d。另设正常对照组(n=10)实施假手术。检测血清IL-10和高迁移率族蛋白(HMGB-1)浓度、脾组织中Sph K1、S1PR1、S1PR3表达情况以及Sph K1酶活性。结果:与对照组比,等渗盐水组大鼠Sph K1 mRNA、蛋白表达和酶活性明显升高,S1PR3 mRNA和蛋白表达也上调;鱼油组血清IL-10和HMGB-1浓度显著降低;Sph K1 mRNA、蛋白表达和酶活性显著受抑制;S1PR3 mRNA和蛋白表达也受抑。结论:n-3 PUFA的PN可通过抑制Sph K1和S1PR3通路改善脓毒症大鼠的炎性反应。
[Abstract]:Objective: to observe whether PNs rich in n-3 polyunsaturated fatty acids (n-3 PUFAs) can affect the inflammatory response through sphingosine kinase 1 (SPH K1) and sphingosine phosphate receptor (S1P). Methods: the external jugular vein cannulation of rats was used to observe whether PNs could affect the inflammatory response of the body through sphingosine kinase 1 (SPH K1) and sphingosine phosphate receptor (S1P). D followed by cecal ligation and perforation, Then they were randomly divided into two groups: isosmotic saline group (intravenous infusion of isosmotic saline), fish oil group (fish oil group) and soybean oil group, then PN support group for 5 days, and normal control group for 5 days. Serum IL-10 and high mobility group protein (HMGB-1) were detected. Results: compared with the control group, the expression of Sph K1 mRNAs, protein expression and enzyme activity of S1PR3 mRNA and protein were significantly increased in Isotonic saline group. The concentration of IL-10 and HMGB-1 in serum of fish oil group significantly decreased SPH K1 mRNAs, and the protein expression and enzyme activity were significantly inhibited. Conclusion the PN of 1: n-3 PUFA can improve the inflammatory response of sepsis rats by inhibiting Sph K1 and S1PR3 pathway.
【作者单位】： 南京大学医学院附属金陵医院(南京军区南京总医院)解放军普通外科研究所;
【基金】：南京军区医学科技创新课题(12Z27)
【分类号】：R459.7
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