基于铈元素的荧光侧向液流免疫层析用于降钙素原测定

发布时间：2018-02-24 08:20

本文关键词： 铈元素侧向液流免疫层析降钙素原微球床旁检测　出处：《基因组学与应用生物学》2016年05期 　论文类型：期刊论文

【摘要】：基于铈元素(Ce)的荧光侧向液流免疫层析(lateral-flow immunoassays,LFIA)技术,开发一种用时短、检测限低的方法,定量测定降钙素原(procalcitonin,PCT),以满足临床检测和诊断的需求。经反向微乳化、氨基修饰、包被Ce,合成Ce微球;其经活化、洗涤,用PCT抗体标记结合和封闭制备微球悬浮剂,固化液相、固定包被和质控抗体、组装和切割等步骤制备PCT试纸条,检测PCT标准品;通过精密度、正确度、回收率、交叉反应和热稳定实验分析该PCT试纸条的临床价值。实验结果显示:用1%含量的Ce微球、100μg标记抗体、Blockmaster TM CE510封闭剂制备的PCT试纸条,采用LFIA法检测PCT,仅需4 min完成检测,大大缩短实验时间;检测限达0.10μg/L;且无边缘和钩状效应、交叉反应;成本低、精密度和准确度高、回收率佳、热稳定性好。因此,本实验基于Ce的LFIA法,成功研发了一种快速、灵敏的PCT床旁定量检测。
[Abstract]:Based on cerium (Ce) fluorescence immunochromatographic lateral flow (lateral-flow immunoassays LFIA) technology, the development of a short time, the method has low detection limit, quantitative determination of procalcitonin (procalcitonin, PCT), in order to meet the needs of clinical examination and diagnosis. By reverse microemulsion, amino modification, package by Ce, the synthesis of Ce microspheres; the activation, washing, labeled with PCT antibody were combined and closed for preparation of microsphere suspension agent, curing liquid, coated and fixed control antibody, assembly and cutting steps of preparation of PCT strip, detection of PCT standard; through the precision, accuracy, recovery, cross the reaction and thermal stability test analysis of the clinical value of the PCT strip. The experimental results show that the Ce microspheres 1% content, 100 g labeled antibody, PCT Blockmaster TM CE510 sealant strip preparation, detected by LFIA PCT, only 4 min to complete the detection, shorten the experiment time limit of detection; It reaches 0.10 micron g/L, and has no edge and hook effect, cross reaction, low cost, high precision and accuracy, good recovery and good thermal stability. Therefore, based on the LFIA method of Ce, this experiment has developed a fast and sensitive PCT bedside quantitative detection.

【作者单位】：中南大学湘雅医学院医学检验系;湖南省肿瘤医院暨中南大学湘雅医学院附属肿瘤医院;
【基金】：长沙市科技局产学研金合作资金专项(K1308118-31)资助
【分类号】：R446.6

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