携氧代血浆扩容及携氧功能初步动物实验研究

发布时间：2018-02-24 21:19

本文关键词： 携氧代血浆多聚人胎盘血红蛋白休克失血扩容复苏肠系膜微循环动物实验 SD大鼠　出处：《中国输血杂志》2016年04期 　论文类型：期刊论文

【摘要】：目的建立大鼠重度失血性休克模型和大鼠失血性休克-肠系膜微循环模型,用以探讨携氧代血浆的扩容和携氧功能。方法将20只SD大鼠失血至全身血容量的60%,造成重度失血性休克大鼠模型后,随机均分为6%羟乙基淀粉(HES)组和携氧代血浆组做相应样品的等容量输注复苏。分别在造模前、休克、输液末以及输液后1 h观测大鼠平均动脉压(MAP)、股动脉取血测定血气,检测组织氧分压(PtO_2)。另取20只大鼠建立失血性休克-肠系膜微循环模型,观察、记录大鼠造模前基础值、休克、输液末以及输液后1 h微血管直径的变化和血流状态。结果 HES组与携氧代血浆组比较,输液末微静脉直径(μm)与微动脉直径(μm)分别为:31.23±4.96 vs 31.86±5.43、19.46±1.47 vs19.40±2.10(P0.05),输液后血流状态均得到恢复;输液后1 h的MAP(mm Hg)为:90.70±3.83 vs 98.90±9.18(P0.05);组织氧分压(mm Hg)为:输液末7.03±1.47 vs 15.32±2.34(P0.05),输液后1 h 5.53±2.39 vs 8.90±2.01(P0.05);2组大鼠血气指标在各时间点相近(P0.05)。结论携氧代血浆能够改善大鼠失血性休克所致的微循环障碍,具有与HES相似的血管扩容作用,并且在大鼠休克复苏初期恢复组织氧分压的效果优于HES。
[Abstract]:Objective to establish a rat model of severe hemorrhagic shock and a rat model of hemorrhagic shock and mesenteric microcirculation. Methods the blood loss of 20 SD rats was increased to 60% of the systemic blood volume, and the model of severe hemorrhagic shock was established in rats. The rats were randomly divided into 6% hydroxyethyl starch (HES) group and oxygen-carrying plasma group for isometric infusion and resuscitation. The mean arterial pressure (map) was measured before, after shock, at the end of infusion and 1 hour after infusion. Blood was collected from femoral artery to determine blood gas. In addition, 20 rats were taken to establish hemorrhagic shock mesenteric microcirculation model. Results the diameter of microvein (渭 m) and the diameter of arteriole (渭 m) were 31.23 卤4.96 vs 31.86 卤5.43 卤19.46 卤1.47 vs19.40 卤2.10P0.05in HES group and those in oxygen-carrying plasma group, respectively. At 1 hour after infusion, the MAP(mm Hg of the rats was: 1: 90.70 卤3.83 vs 98.90 卤9.18m P0.05A; the tissue oxygen partial pressure was 7.03 卤1.47 vs 15.32 卤2.34p0.05n; the blood gas index of rats in the group of 1 h after infusion was 5.53 卤2.39 vs 8.90 卤2.01P0.05U. Conclusion the oxygen-carrying substitute plasma can improve the microcirculation disturbance caused by hemorrhagic shock in rats. The effect of vasomascularization was similar to that of HES, and the effect of recovering tissue oxygen partial pressure in the early stage of shock resuscitation was better than that of HES.
【作者单位】：中国医学科学院北京协和医学院输血研究所;
【基金】：四川省科技支撑计划项目(2013SZ0059,2014SZ0123) 四川省国际科技合作与交流研究计划(2014HH0065)
【分类号】：R605.971;R457.1

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