2010-2014年上海市志贺菌菌群分布及耐药性分析

发布时间：2018-02-25 09:37

本文关键词： 志贺菌耐药分析菌群分布耐药基因　出处：《现代预防医学》2016年05期 　论文类型：期刊论文

【摘要】：目的探讨近五年来上海市肠道门诊腹泻患者中分离到的志贺菌菌群分布及其耐药特点,为细菌性痢疾临床治疗和预防控制提供依据。方法采用改良的K-B法检测志贺菌对抗菌药物的耐药性,并应用PCR法对耐药基因进行检测。结果共分离出382株志贺菌,其中宋内志贺菌243株(占63.6%),福氏志贺菌139株(占36.4%)。菌株对萘啶酸、链霉素、氨苄西林、四环素、复方新诺明和磺胺异恶唑的耐药率依次为97.4%、96.1%、84.4%、77.7%、77.2%和67.8%,其中92.7%的菌株至少对3种及以上的抗生素产生了耐药,6.3%的菌株对10~13种抗生素产生了耐药,且存在对氟喹诺酮类和第三、四代头孢的耐药株。结论志贺菌耐药情况严重,且福氏和宋内两种志贺菌对部分抗菌药物的抗药性差别较大,临床医师应根据菌群鉴定及药物敏感试验结果合理选择抗菌药物。
[Abstract]:Objective to investigate the distribution and drug resistance of Shigella isolated from diarrhea patients in outpatient department of intestinal tract in Shanghai in recent five years. Methods A modified K-B method was used to detect the resistance of Shigella to antimicrobial agents, and PCR method was used to detect the resistance of Shigella to antimicrobial agents. Results 382 strains of Shigella were isolated. Among them, 243 strains of Shigella sonnei (63.6%), 139 strains of Shigella flexneri (36.4%). The drug resistance rates of compound sulfamethoxazole and sulfamethoxazole were 97.4% and 77.72%, respectively, and 77.2% and 67.8%, respectively. Among them, 92.7% strains showed resistance to at least 3 antibiotics and more than 3 antibiotics, and to 103 antibiotics, and to fluoroquinolones and to fluoroquinolones. Conclusion the drug resistance of Shigella is serious, and the resistance of Shigella flexneri and Shigella to some antimicrobial agents is quite different, so the clinicians should select antibiotics reasonably according to the results of microflora identification and drug sensitivity test.
【作者单位】：东华大学化学化工与生物工程学院;上海市疾病预防控制中心;上海交通大学公共卫生学院;
【基金】：上海市卫生计生系统优秀学科带头人培养计划(XBR2011051) 上海市公共卫生重点学科卫生微生物学(12GWZX0801) 致病性新型分子血清分型系统的建立(2013ZX10004216-001-004)
【分类号】：R446.5

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