核酸检测技术在不同血液安全筛查模式的应用分析

发布时间：2018-02-28 14:22

  本文关键词： 酶联免疫试验 核酸扩增技术 检测模式　出处：《中国输血杂志》2016年07期 　论文类型：期刊论文

【摘要】：目的比较核酸检测技术(NAT)在无偿献血不同血液安全筛查模式下阳性反应率。方法在2010年1月1日-2013年8月31日期间,采用2遍ELISA检测,无反应性样本采用Cobas Taqsceen MPX Test进行1遍NAT;2013年9月1日-2014年9月29日期间,采用1遍ELISA检测,无反应性样本采用Cobas Taqsceen MPX Test进行1遍NAT;2014年9月29日-2015年8月31日期间,采用1遍ELISA检测,无反应性样本采用Cobas Taqsceen MPX Test v2.0(MPX v2.0)进行1遍NAT。对NAT混检阳性pool采用MPX v2.0拆分单检,并鉴别病毒种类;对ELISA检测抗-HIV无反应性、HIV RNA有反应性的献血者定期进行追踪分析,观察有无血清学转换,以确定感染状态。结果3个阶段共完成NAT标本422 667份,混检阳性898pools,阳性率0.21%,其中HBVDNA混检阳性率为0.209%(893/422 667),HCVRNA混检阳性1例(1/422 667),HIVRNA混检阳性4例(4/422 667);拆分单检总阳性率为0.126%(551/422 667),拆分阳性率为61.35%(551/898),拆分后单检HBVDNA阳性545例,HCVRNA阳性2例,HIVRNA阳性4例。对HIVRNA阳性样本进行定期追踪分析,4例献血者均在献血后3个月内发生血清学阳转,确定均为窗口期感染HIV。3个阶段采用不同的检测模式,NAT总阳性反应率无显著性差异;拆分单检阳性反应率Ⅲ阶段高于Ⅰ阶段(P0.05)。结论 NAT检测的总阳性反应率与检测模式无关;应用NAT可降低输血风险,尤其对HBV窗口期感染的阳性检出率较高。
[Abstract]:Objective to compare the positive reaction rate of nucleic acid test (Nat) in different blood safety screening modes of blood donation. Methods from January 1st 2010 to August 31st 2013, ELISA was used twice. The non-reactive samples were performed once with Cobas Taqsceen MPX Test; during the period from September 1st 2013 to September 29th 2014, with one ELISA test; with Cobas Taqsceen MPX Test; and with Cobas Taqsceen MPX Test with the period September 29th 2014 to September 29th 2014. ELISA was used once, and Cobas Taqsceen MPX Test v2.0MPX v2.0 was used to detect the non-reactive samples. MPX v2.0 was used to separate the NAT positive pool and identify the virus types. In order to determine the status of infection, the blood donors who were tested by ELISA for anti-HIV / RNA reactivity were regularly followed up and analyzed. Results 422,667 NAT samples were collected in three stages. The positive rate of HBVDNA mixed detection was 0.209 and 0.209 respectively. One case was positive for 1 / 422 667HIV RNA, 4 cases were positive for HIV RNA, the total positive rate was 0.1266 551 / 422 667G, the positive rate of HBVDNA was 61.35% 551% 8987.After resolution, 545 cases were positive for HBVDNA and 545 cases were positive for HIV RNA and HBVDNA respectively, respectively. The total positive rate was 0.1266 551 / 422 667G, and the positive rate of HBVDNA was 61.35% 551% 8988.The positive rate of HBVDNA was 551% 8988.The positive rate of HBVDNA was 545 cases after the resolution, and the total positive rate was 0.1266 551 / 422,667%. The total positive rate of HBVDNA was 0.126 551% 898%. 4 cases were positive. The positive samples of HIVRNA were regularly followed up. 4 cases of blood donors all developed serological positive conversion within 3 months after blood donation. There was no significant difference in the total positive reaction rate of Nat in all the stages of window infection with different detection modes, and the positive reaction rate in stage 鈪，

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