Nafion修饰碳纤维微电极在抗坏血酸共存下选择性测定去甲肾上腺素

发布时间：2018-03-07 06:41

本文选题：Nafion　切入点：碳纤维电极　出处：《分析测试学报》2016年06期 　论文类型：期刊论文

【摘要】：制备了碳纤维微电极,将洁净的碳纤维微电极浸入Nafion溶液中,采用电沉积的方法制得Nafion修饰碳纤维微电极。采用循环伏安法(CV)、差分脉冲伏安法(DPV)研究了去甲肾上腺素(NE)和抗坏血酸(AA)在电极上的电化学行为。结果表明:在最优条件下制备的Nafion修饰电极能完全屏蔽AA的电化学响应,而对NE仍表现出良好的电化学响应。修饰电极能在1.0 mmol/L AA的共存下选择性地测定NE,采用DPV进行检测,NE的氧化峰电流与其浓度在1.0×10~(-6)~1.0×10~(-4)mol/L范围内呈良好的线性关系,相关系数(r~2)为0.991 2,检出限(S/N=3)为8.6×10~(-7)mol/L。利用该方法测定了模拟样品中NE的含量,平均加标回收率为101.6%。该电极的重现性和稳定性良好,且具有良好的灵敏度和选择性,有望用于复杂生物环境中NE浓度的检测。
[Abstract]:Carbon fiber microelectrode was prepared, and the clean carbon fiber microelectrode was immersed in Nafion solution. Carbon fiber microelectrode modified by Nafion was prepared by electrodeposition. The electrochemical behavior of NE) and ascorbic acid were studied by cyclic voltammetry and differential pulse voltammetry. The electrochemical response of AA can be completely shielded by the Nafion modified electrode prepared under the optimal conditions. However, the modified electrode can selectively determine NEs in the presence of 1.0 mmol/L AA, and the oxidation peak current of NE detected by DPV has a good linear relationship with its concentration in the range of 1.0 脳 10 ~ (-1) ~ (-6) ~ (-1) mol / L ~ (-1) ~ (-1) mol 路L ~ (-1) 路L ~ (-1) ~ (-1) 路L ~ (-1) ~ (-1) ~ (-1) 路L ~ (-1) ~ (-1). The correlation coefficient was 0.991 2, and the detection limit was 8.6 脳 10 ~ (-10) ~ (-7) mol 路L ~ (-1). The method was used to determine the content of NE in the simulated sample. The average recovery rate was 101.6%. The electrode had good reproducibility and stability, and had good sensitivity and selectivity. It is expected to be used for the detection of NE concentration in complex biological environment.
【作者单位】：中南民族大学药学院;
【基金】：国家自然科学基金项目(21205144) 中南民族大学学术团队(XTZ15013);中南民族大学民族药学十二五实验教学示范中心资助
【分类号】：O657.1;R446

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