基于纳米复合材料构建特异性检测人血清中唾液酸转移酶的电化学免疫传感器的研究

发布时间：2018-03-09 22:23

本文选题：免疫传感器　切入点：普鲁士蓝　出处：《重庆医科大学》2015年硕士论文　论文类型：学位论文

【摘要】：近年来,电化学免疫传感器因具有高特异性、高灵敏性和低成本等特点而广泛应用于临床诊断和生物样品分析领域,己成为一种生物标志物的理想分析技术。随着纳米技术的发展,电催化的纳米复合物和纳米金属材料以其独特的电化学特性和良好的生物相容性,在电化学免疫传感器的构建领域得到广泛应用。基于以上优势,本文将金纳米粒子(AuNPs)负载到以普鲁士蓝(PB)为基础的杂化纳米复合物膜表面的方法,构建了一种用于灵敏性检测p-半乳糖αα-2,6唾液酸转移酶I (ST6Gal-I)的新型电化学免疫传感器。以普鲁士蓝为基础的杂化纳米复合物由普鲁士蓝(PB)、氧化石墨烯(GO)、多壁碳纳米管(MWCNTs)、PTA (3,4,9,10-茈四甲酸二酐的衍生物,即PTC-NH2)和壳聚糖(CS)四种物质组成,并作为该免疫传感器的电极修饰材料。在壳聚糖和PTA表面丰富的氨基基团的协助下,通过Au和-NH2之间的强相互作用,将AuNPs吸附在纳米复合物表面,进而提供足够大的表面积来固定大量的ST6Gal-I抗体(anti-ST6Gal-I)。该免疫传感器检测方法中,PB可作为一种具有生物相容性和电催化活性的氧化还原探针提供检测的电信号,并利用循环伏安法和示差脉冲伏安法进行检测表征。在最优化的实验条件下,该免疫传感器在0.01到250 ng-mL-1检测范围内呈现良好的线性响应,且最低检测限为pg· mL-1(S/N=3)。此外,这种分析方法成功应用于人血清样品中的ST6Gal-I的检测分析。因此,本文构建的免疫传感器能够实现对ST6Gal-I进行特异性强且高灵敏的检测,并在未来的临床和实验分析方面有着潜在应用前景。
[Abstract]:In recent years, electrochemical immunosensors have been widely used in clinical diagnosis and biological sample analysis due to their high specificity, high sensitivity and low cost. With the development of nanotechnology, electrocatalytic nanocomposites and nanometallic materials have unique electrochemical properties and good biocompatibility. It is widely used in the field of electrochemical immunosensor. Based on the above advantages, the gold nanoparticles AuNPs were loaded on the surface of hybrid nanocomposite membrane based on Prussian blue blue monolayer (PBB). A novel electrochemical immunosensor for the sensitive detection of pgalactose 伪-伪 -2- 6 sialic acid transferase I (ST6Gal-I) was constructed. The hybrid nanocomposites based on Prussian blue were composed of Prussian blue, graphene oxide and multiwalled carbon nanocomposites. Derivatives of tetracarboxylic anhydride, PTC-NH2) and chitosan CS2) were used as electrode modification materials of the immunosensor. With the assistance of chitosan and abundant amino groups on PTA surface, the strong interaction between au and -NH2 was obtained. AuNPs was adsorbed on the surface of nanocomposites, The immunosensor provides a large enough surface area to immobilize a large number of ST6Gal-I antibodies, anti-ST6Gal-IK. The immunosensor can provide electrical signals for detection as a biocompatible and electrocatalytic redox probe. Cyclic voltammetry and differential pulse voltammetry were used to detect and characterize the immunosensor. Under the optimized experimental conditions, the immunosensor showed a good linear response in the range of 0. 01 to 250 ng-mL-1, and the lowest detection limit was PG 路ml ~ (-1) S / N ~ (3 +). This method has been successfully applied to the detection and analysis of ST6Gal-I in human serum samples. Therefore, the immunosensor constructed in this paper can detect ST6Gal-I with strong specificity and high sensitivity. And it has potential application prospect in clinical and experimental analysis in the future.
【学位授予单位】：重庆医科大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R446.1;TP212.3

【相似文献】