阴离子交换色谱-荧光检测法测定人体血清中酪氨酸及其代谢产物

发布时间：2018-03-15 02:34

本文选题：离子色谱-荧光检测法　切入点：人体血清　出处：《分析科学学报》2016年02期 　论文类型：期刊论文

【摘要】：建立了阴离子交换色谱-荧光检测法(AEC-FLD)同时测定人体血清中的酪氨酸(Tyr)、对羟苯基乳酸(PHPLA)和对羟苯基丙酸(PHPA)的分析新方法。样品经乙腈处理沉淀蛋白,离心后取上清液直接进样检测。采用IonPac AG21保护柱(50×2mm)、IonPac AS21分析柱(250×2mm),流动相为4mmol/L NaOH溶液,流速为0.45mL/min,荧光检测器的激发波长和发射波长分别为277nm和340nm。优化条件下三种物质能得到良好的分离和检测。Tyr、PHPLA和PHPA的线性范围分别为0.30~25mg/L、0.025~2mg/L和0.025~2mg/L,相关系数在0.9998~0.9999之间,检出限(S/N=3)分别为0.024mg/L、0.020mg/L和0.019mg/L。三个水平的加标回收率在72.6%~99.1%之间,其相对标准偏差均小于6.62%。该方法简单、快速、准确,可用于实际样品的测定。
[Abstract]:A new method for the simultaneous determination of tyrosine tyrosine tyrosine, p-hydroxyphenyl lactic acid PHPLA and p-hydroxyphenyl propionate PHPA in human serum by anion exchange chromatography and fluorescence detection was established. The protein was precipitated by acetonitrile treatment. After centrifugation, the supernatant was directly injected into the sample. The IonPac AG21 protection column 50 脳 2mm Pac AS21 was used for the analysis of 250 脳 2mm Pac column. The mobile phase was 4 mmol / L NaOH solution, the mobile phase was 4 mmol / L NaOH solution, the mobile phase was 4 mmol / L NaOH solution. At a flow rate of 0.45 mL / min, the excitation and emission wavelengths of the fluorescence detector are 277nm and 340 nm respectively. Under the optimized conditions, the linear range of the separation and detection of the three substances is 0.30 ~ 25mg / L 0.025mg / L 0.025mg / L and 0.0252mg / L, respectively, and the correlation coefficient is between 0.9998 and 0.9999. The detection limit is 0.024 mg / L 0.020 mg / L and 0.019 mg / L. the recoveries of the three levels are between 72.6% and 99.1%, and the relative standard deviations are less than 6.62%. The method is simple, rapid and accurate, and can be used for the determination of practical samples.
【作者单位】：南昌大学化学学院;南昌大学分析测试中心;
【基金】：国家自然科学基金(No.21465017) 江西省自然科学基金(No.20122BAB203013)
【分类号】：O657;R446.11

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