血培养阳性标本直接药敏试验临床应用价值的评估

发布时间：2018-03-15 23:28

本文选题：血流感染　切入点：血培养　出处：《中华医院感染学杂志》2016年10期 　论文类型：期刊论文

【摘要】：目的研究血培养阳性标本直接药敏结果的可靠性和方法的可行性,为临床血流感染的快速治疗提供指导。方法选取医院2014年1-3月的64份血培养阳性标本,其中革兰阳性菌33份,革兰阴性菌31份,取培养液直接涂片革兰染色,观察细菌形态及染色;用培养液原液分别直接涂布和离心后的上清液涂布M-H平板,根椐染色结果选用不同药敏纸片作纸片扩散法(K-B法)药敏试验,同时按常规操作接种平板分离菌株后作标准药敏试验,分别比较直接药敏及上清药敏与标准药敏结果的符合程度和两种方法之间的差异。结果测试的64份标本,直接药敏及上清药敏与标准药敏结果相比,结果的误差率均在可接受范围内,与标准药敏结果相比,原液直接药敏与离心后上清药敏结果的符合率之间差异均无统计学意义。结论血培养直接药敏与标准药敏结果有很好的符合率,培养液是否离心对结果影响差异无统计学意义,直接药敏缩短了检验时间,减少了抗菌药物的滥用。
[Abstract]:The feasibility and reliability of positive specimens for drug sensitivity test to study blood culture, provide guidance for rapid clinical treatment of bloodstream infection. Methods 64 specimens were positive blood hospital in 2014 1-3 months of training, including 33 gram positive bacteria, gram negative bacteria were cultured in liquid 31, direct smear gram stain and the observation of bacterial morphology and staining; coated M-H plate with culture medium supernatant liquid were directly coated and after centrifugation, according to the staining results of different drug susceptibility for disk diffusion method (K-B method) and drug sensitivity test, routine inoculation plate isolates after standard drug sensitivity test, the difference between the direct comparison respectively. Drug sensitivity and drug sensitivity and drug sensitivity was the coincidence degree and two methods. The test results of 64 specimens, compared to direct drug sensitivity and drug sensitivity was with the standard drug sensitivity results, the error rate in the Within the acceptable range, compared with the standard drug sensitivity results, with direct drug sensitivity and liquid after centrifugation supernatant drug sensitivity rate between groups were not statistically significant. Conclusion blood culture direct drug sensitivity and drug sensitivity results in line with the standard rate is very good, whether the influence of centrifugation on liquid culture showed no significant difference, directly the drug sensitivity test to shorten the time, reduce the abuse of antibiotics.

【作者单位】：江苏省中医药研究院检验科;江苏省中医药研究院内分泌学研究室;
【基金】：江苏省自然科学基金资助项目(BK20131037)
【分类号】：R446.5

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