流式细胞仪检测P-selectin和t-PA的方法学评价及临床应用

发布时间：2018-03-17 03:27

本文选题：流式细胞仪　切入点：方法学评价　出处：《重庆医学》2016年34期 　论文类型：期刊论文

【摘要】：目的对FACSArray流式细胞仪检测P选择素(P-selectin)、组织型纤溶酶原激活物(t-PA)进行方法学性能评价,并观察其与冠心病各亚组的相关性。方法用FACSArray流式细胞仪检测系统测定P-selectin、t-PA的精密度、准确度,对灵敏度、干扰试验等分析性能进行验证和评价。同时,对患有冠心病的患者血清P-selectin、t-PA浓度进行检测,观察其血清含量与冠心病的相关性。结果 P-selectin、t-PA的批内和批间变异系数分别为4.23%~9.00%、4.63%~11.00%和2.20%~2.90%、6.30%~12.20%;P-selectin、t-PA的相对偏倚分别为2.71%~5.28%和1.23%~4.16%,检测灵敏度为16.20ng/L和1.21ng/mL。高浓度的三酰甘油、血红蛋白和胆红素对P-selectin测定时的干扰率分别为14.65%、13.89%和10.94%,对t-PA测定时的干扰率分别为15.65%、13.23%和12.94%;各冠心病亚组P-selectin的浓度范围为(54 020.1±3 390.1)~(83 189.8±7 226.3)ng/L,t-PA的浓度范围为(9.59±2.13)~(28.72±2.15)ng/mL,与对照组相比,差异有统计学意义(P0.05)。结论FACSArray流式细胞仪检测系统检测血清P-selectin、t-PA的分析性能符合规定的性能指标,能够满足实验室认可的需要。
[Abstract]:Objective to evaluate the methodological performance of P-selectinus and tissue plasminogen activator (t-PA) detected by FACSArray flow cytometry, and to observe its correlation with various subgroups of coronary heart disease. Methods the precision of P-selectint-PA was measured by FACSArray flow cytometry. Accuracy, sensitivity, interference test, and so on were verified and evaluated. Serum P-selectint-PA levels were also measured in patients with coronary heart disease. Results the coefficients of variation of P-selectint-PA were 4.23ng / ml and 1.23ng/ mL, respectively. The relative bias of P-selectint-PA was 2.715.28% and 1.23ng / mL, respectively. The relative bias of P-selectint-PA was 16.20 ng / L and 1.21 ng / mL, respectively, and the relative bias of P-selectint-PA was 2.715.28% and 1.234.16.The sensitivity was 16.20 ng / L and 1.21 ng / mL respectively, and the relative bias of P-selectint-PA was 2.715.28% and 1.234.16.The sensitivity was 16.20 ng / L and 1.21 ng / mL, respectively. The interference rates of hemoglobin and bilirubin on P-selectin were 13.89% and 10.94, respectively, and the interference rates on t-PA were 15.65 ~ 13.23% and 12.94 respectively. The concentration range of P-selectin in each coronary heart disease subgroup was 54 020.1 卤3 390.1 ~ 83 189.8 卤7 226.3 ng / L ~ (t-PA) in the range of 9.59 卤2.13 ~ (-1) 卤2.15 ng 路mL ~ (-1), compared with the control group. Conclusion the analytical performance of serum P-selectint-PA by FACSArray flow cytometry can meet the requirements of laboratory accreditation.
【作者单位】：贵州医科大学附属省人民医院检验中心;贵州医科大学基础医学院;
【基金】：贵州省省长资金临床应用课题专项研究项目[黔省专合字(2012)117号] 贵州省卫生厅科学技术基金项目(gzwkj2012-1-129);贵州省卫生厅立项资助项目(gzwkj2012-1-062)
【分类号】：R446.1;R541.4

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