6292份脐带血的微生物检测结果分析

发布时间：2018-03-21 21:32

本文选题：脐带血　切入点：微生物污染　出处：《重庆医学》2016年35期 　论文类型：期刊论文

【摘要】：目的探讨脐带血采集和处理过程中微生物污染的概率、种类,并分析脐带血收集相关因素和检测方法对培养结果的影响。方法抽取2011~2014年脐带血分离后的血浆-红细胞悬液,分别注入需氧和厌氧培养瓶,使用BACTEC9000系列全自动血液培养系统培养5d,结果阳性者做废弃处理。对阳性标本进行病原菌鉴定,同时比较不同生产方式、采集者、采集量、采集方式、处理前时间、培养体积、延迟上机时间及培养瓶种类对微生物污染率的影响。结果 6 292份被检脐带血中阳性标本131份,污染率为2.1%。病原菌以大肠埃希菌最为常见,占23.9%,其次为凝固酶阴性葡萄球菌,占20.9%。自然分娩者脐带血标本、县市级医院采集标本、采集量低于80mL的标本阳性检出率升高,培养体积由10mL提高为20mL后阳性检出率上升,差异均有统计学意义(P0.05)。结论脐带血微生物污染率与生产方式、采集者及采集量相关,增加培养体积可提高检测灵敏度。
[Abstract]:Objective to investigate the probability and species of microorganism contamination in the process of umbilical cord blood collection and treatment, and to analyze the influence of the related factors of cord blood collection and detection methods on the culture results. Methods the plasma erythrocyte suspension isolated from umbilical cord blood from 2011 to 2014 was extracted. Aerobic and anaerobic culture flasks were injected into aerobic and anaerobic culture flasks respectively, and BACTEC9000 series automatic blood culture system was used to culture for 5 days. The results showed that the positive samples were treated with waste. The pathogens of the positive specimens were identified, and the different modes of production, the gatherer and the amount of collection were compared. Results 131 positive samples from 6 292 umbilical cord blood samples were collected, and the contamination rate was 2.1%. Escherichia coli was the most common pathogen. 23.9%, followed by coagulase-negative staphylococcus, 20.9.The positive rate of samples collected from natural delivery patients and county and municipal hospitals was higher than that of 80mL, and the positive rate of culture volume from 10mL to 20mL increased. Conclusion the rate of microorganism contamination in umbilical cord blood is related to the mode of production, collector and quantity of collection, and the sensitivity of detection can be improved by increasing the volume of culture.
【作者单位】：中国医科大学附属盛京医院辽宁省脐血库;
【分类号】：R446.5

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