铁蛋白时间分辨荧光免疫层析法的建立及临床应用

发布时间：2018-03-25 08:17

  本文选题：铁蛋白　切入点：时间分辨荧光免疫分析法　出处：《现代免疫学》2016年01期

【摘要】：建立铁蛋白(FER)时间分辨荧光免疫层析检测法(TRFIA-POCT)并进行临床应用。将FER抗体和兔IgG包被在硝酸纤维素膜(NC膜)上,以荧光微球标记抗FER单克隆抗体及兔IgG分别作为检测线和质控线制备荧光免疫层析试纸条。FER-TRFIA-POCT的灵敏度为0.5ng/ml;批内CV为3.2%~4.6%,批间CV为3.8%~5.2%;平均回收率为100.5%;热稳定性好;与电化学发光分析技术(ECLIA)比对,相关系数达0.8898。正常参考值范围男性为90~350ng/ml,女性为30~260ng/ml。本法建立的FER-TRFIA-POCT是一个快速高灵敏和可靠的检测。
[Abstract]:A time-resolved fluorescence immunochromatographic assay (TRFIA-POCTT) was established for the detection of ferritin. FER antibody and rabbit IgG were coated on the nitrocellulose membrane (NC). The sensitivity of FER-TRFIA-POCT was 0.5 ng / ml using fluorescent microspheres labeled monoclonal antibody against FER and rabbit IgG as detection line and quality control line, respectively. The sensitivity of FER-TRFIA-POCT was 0.5 ng / ml, the CV was 3.2g / ml, and the interassay CV was 3.80.2.The average recovery was 100.5 and the thermal stability was good. Compared with ECLIA, the correlation coefficient was 0.8898.The normal reference value range was 90 ~ 350ng / ml for men and 30 ~ 260ng / ml for women. The FER-TRFIA-POCT established by this method was a rapid, sensitive and reliable method.
【作者单位】： 江苏省原子医学研究所江苏省分子核医学重点实验室卫生部核医学重点实验室;江阴市人民医院检验科;
【基金】：无锡市卫生局科研项目(MS201530)
【分类号】：R446.6
，

本文编号：1662275