Tat-LK15运载nNOS siRNA治疗慢性炎性疼痛的实验研究

发布时间：2018-03-25 09:31

本文选题：细胞穿透肽　切入点：神经型一氧化氮合酶　出处：《实用医学杂志》2016年08期

【摘要】：目的:慢性疼痛如炎性疼痛的基因治疗缺乏安全且高效的载体,本实验探讨了细胞穿透肽Tat-LK15介导si RNA干扰大鼠脊髓背角n NOS表达进而治疗慢性炎性疼痛的可行性。方法:参照前期实验,检测鞘内注射Tat-LK15/si RNA对完全弗氏佐剂(CFA)炎性痛模型大鼠脊髓背角(SCDH)n NOS蛋白表达的影响,并测定鞘内注射此复合物后炎性痛大鼠机械缩足反射阈值(MWT)及热缩足反射持续时间(TWD)的变化。结果:Tat-LK15/si RNA注射后3 d,炎性疼痛大鼠SCDH n NOS蛋白水平的表达下降51%(P0.01),Tat-LK15/sc RNA注射后n NOS的表达无明显变化。Tat-LK15/si RNA鞘内注射后3、7、14及21d炎性痛大鼠的MWT显著增高,TWD显著缩短(P0.01),Tat-LK15/sc RNA无此治疗作用。结论:TatLK15可有效运载si RNA抑制CFA所致慢性炎性痛大鼠n NOS的过度表达,从而对炎性疼痛大鼠具有治疗作用。
[Abstract]:Objective: gene therapy for chronic pain such as inflammatory pain lacks a safe and efficient vector. The aim of this study was to investigate the feasibility of interfering the expression of si RNA with the expression of n NOS in the dorsal horn of the spinal cord of rats and to treat chronic inflammatory pain by cell penetrating peptide (Tat-LK15). The effect of intrathecal injection of Tat-LK15/si RNA on the expression of NOS protein in spinal dorsal horn of rats with inflammatory pain induced by complete Freund's adjuvant was detected. The expression of SCDH n NOS protein in inflammatory pain rats was reduced by 51% Tat-LK15% si RNA 3 days after injection of Tat-LK15% si RNA. Results the expression of Tat-LK15sc RNA in inflammatory pain rats was decreased by 51g / L P0.01Tat-LK15sc RNA after intrathecal injection of the compound. There was no significant change in the expression of n NOS after injection. Tat-LK15 / si RNA intrathecal injection increased MWT significantly in rats with inflammatory pain on the 3rd day and 21st day after injection. Tat-LK15 / sc RNA has no effect on this treatment. Conclusion: Tat-LK15 can effectively transport si RNA to inhibit chronic inflammatory pain induced by CFA in rats. N NOS overexpression, So it has therapeutic effect on inflammatory pain rats.
【作者单位】：广东省中山市博爱医院麻醉科;广州中医药大学第二附属医院;广州军区广州总医院麻醉科;
【基金】：国家自然科学基金资助项目(编号:81100817,81371233)
【分类号】：R402

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