某食用白酒对大鼠血糖的影响及其机制的探讨

发布时间：2018-03-29 08:41

本文选题：食用白酒　切入点：血糖　出处：《贵阳医学院》2015年硕士论文

【摘要】：目的:对SD大鼠进行灌胃,探讨摄入某品牌两种浓度食用白酒(54°和38°)后,不同时长及不同剂量对大鼠空腹血糖、胰岛B细胞表达胰岛素(Ins)、A细胞表达胰高血糖素(Glu)及血清Ins及C肽(C-P)的改变,以期为探索科学健康饮酒提供一定实验依据。方法:正常成年雄性SD大鼠90只,随机分为实验组(experiment group,EG)及正常对照组(normal control group,NCG)。(1)54°实验组45只:分为4周组(4w)、8周组(8w)、12周组(12w),其中每周组均分为低剂量组(low dose group,L组,0.8ml/kg·day)、中剂量组(middle dose group,M组,1.6ml/kg·day)、高剂量组(high dose group,H组,2.4ml/kg·day),每个剂量组5只大鼠。(2)38°实验组30只:分为4w、12w;各周组又分为三个剂量组,即L组、M组、H组,每个剂量组5只大鼠;(3)各周组大鼠均有5只作正常对照。实验组大鼠予相应食用白酒灌胃,每日11:00Am及5:00Pm各灌胃一次,正常对照组不予处理。分别于第4w末,第8w末,第12w末测空腹血糖,取胰尾组织及血清,采用放射免疫法、免疫组化SABC法、图像分析及形态计量法进行研究。结果:1.54°组:(1)各实验组大鼠空腹血糖水平与正常对照组比较,差异无统计学意义(P0.05)。(2)血清Ins放免结果显示:仅8w(H)组、12w(L)组大鼠血清Ins水平与正常对照组比较有所上升,差异具有统计学意义(P0.05),其余各组未见明显变化。(3)免疫组化结果显示:光镜下,Ins、Glu免疫反应阳性产物呈棕黄至棕褐色细颗粒状,位于胞质内。①与正常对照组比较,除8w(H)组和12w(L)组稍有升高外(P0.05),余实验各组Ins阳性细胞的免疫染色强度及平均光密度的差异无统计学意义(P0.05)。②与正常对照组比较,除12w(L)组稍有升高(P0.05)外,余实验各组Glu阳性细胞的免疫染色强度及平均光密度的差异无统计学意义(P0.05)。(4)形态学计量结果显示:与正常组比较,各实验组Ins阳性细胞及Glu阳性细胞的面数密度(NA)均未见明显变化(P0.05)。2.38°组:(1)各实验组大鼠空腹血糖水平与正常对照组比较,差异无统计学意义(P0.05)。(2)血清C肽放免结果显示:与正常对照组比较,仅12w(L)组血清C肽水平稍有升高(P0.05),余各实验组未见明显改变。(3)免疫组化结果显示:各实验组Ins阳性细胞、Glu阳性细胞的分布、数量及免疫染色强度与正常组比较未见明显变化;①各实验组Ins阳性细胞、Glu阳性细胞平均光密度值未见明显改变(P0.05);②与正常组比较,各实验组Ins阳性细胞、Glu阳性细胞的面数密度(NA)亦未见明显改变(P0.05)。结论:某品牌54°及38°食用白酒灌胃,在本实验设定的剂量和时程内,大鼠血糖未见明显异常;对胰岛B细胞表达Ins、胰岛A细胞表达Glu和面数密度、血清Ins及C肽无明显影响。
[Abstract]:Objective: to study the effects of different time and dosage on fasting blood glucose of SD rats after ingesting two different concentrations of liquor (54 掳and 38 掳). In order to provide some experimental evidence for the study of scientific and healthy drinking, the expression of glucagon Glua and serum Ins and C-Pin in islet B cells were determined. Methods: 90 adult male Sprague-Dawley rats were included in this study. The experiment group was randomly divided into two groups: experimental group (n = 45) and normal control group group (n = 45). The experimental group (n = 45) was divided into 4 weeks group (n = 45) and a control group (n = 45). The experimental group was divided into 4 weeks group, 8 weeks group, 8 weeks group and 12 weeks group, respectively. Each week group was divided into low dose group group group (0. 8 ml / kg), middle dose group group (middle dose group M group) and high dose group group (1. 6 ml / kg), and the high dose group group group was divided into two groups: low dose group group (0. 8 ml / kg) and middle dose group group M group (1. 6 ml / kg). The experimental group (n = 30) was divided into 4 weeks and 12 weeks, and each week group was divided into three dose groups. There were 5 rats in each week group as normal control group. Rats in the experimental group were given corresponding drinking liquor, 11:00Am and 5:00Pm were given orally once a day, and the normal control group was not treated at the end of 4 weeks. Fasting blood glucose was measured at the end of the 8th week and the end of the 12th week. The pancreatic tail tissue and serum were collected. Radioimmunoassay and immunohistochemical SABC method were used. Image analysis and morphometry were used to study the results. Results the fasting blood glucose levels of the rats in the 1: 1.54 掳group were compared with those in the normal control group. The results of serum Ins radioimmunoassay showed that the serum Ins level of rats in the group of only 8 weeks of HH) was higher than that in the control group at 12 ws (P < 0.05), and the serum Ins level of the rats in the control group was significantly higher than that in the control group (P < 0.05). The difference was statistically significant (P 0.05), but there was no significant change in the other groups. The results of immunohistochemistry showed that the immunoreactive products of Instrol Glu were brown and brown, and they were located in cytoplasm of 1. 1 as compared with the normal control group. Except for the 8w HG and 12wL) groups, the immunoreactivity and average optical density of the Ins positive cells in the other groups were not significantly different from those in the normal control group, except for the 12 wk L) group, which was slightly higher than that of the control group (P 0.05), and the other two groups had no significant difference in the immunostaining intensity and the average optical density (P 0.05), except for the 12 wk L) group, which had a slight increase (P 0.05). There was no significant difference in the immunostaining intensity and average optical density of Glu positive cells in the remaining experimental groups. The results of morphological measurement showed that: compared with the normal group, there was no significant difference between the two groups. There was no significant change in the number density of Ins positive cells and Glu positive cells in each experimental group. (P0.05U. 2.38 掳group) the fasting blood glucose levels of rats in each experimental group were compared with those of the normal control group. There was no significant difference in serum C-peptide radioimmunoassay results: compared with the normal control group, there was no significant difference in serum C-peptide radioimmunoassay. The level of serum C-peptide was slightly increased in 12 weeks (P < 0.05), but no significant change was found in the other experimental groups. The immunohistochemical results showed that the distribution of Ins positive cells in each experimental group was not obvious, and the distribution of Glu-positive cells in the other experimental groups was higher than that in the control group (P < 0.05). There was no significant change in the number and the intensity of immunostaining compared with the normal group. The average optical density of the Ins positive cells in each experimental group was not significantly changed compared with the normal group, and there was no significant change in the average optical density of the Glu-positive cells in each experimental group compared with the normal group. The surface number density of Ins positive cells in each experimental group was not significantly changed (P 0.05). Conclusion: a brand of 54 掳and 38 掳edible liquor was perfused by stomach, and there was no obvious abnormality of blood glucose in rats during the time course and dose set in this experiment. [WT5HZ] [WT5BZ] [WT5 "BZ] [WT5" BZ] [WT5 "BZ] [WT5" BZ]. The expression of Ins in islet B cells, Glu expression and surface number density in islet A cells, serum Ins and C-peptide were not significantly affected.
【学位授予单位】：贵阳医学院
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R446.6

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