不同液体复苏方式对失血性休克大鼠肠道影响的研究

发布时间：2018-04-03 04:35

本文选题：失血性休克　切入点：复苏　出处：《石河子大学》2015年硕士论文

【摘要】：目的探讨不同液体复苏方式对失血性休克大鼠肠道形态、炎症反应和免疫功能及细胞凋亡的影响。方法本研究将60只SD大鼠制作成失血性休克模型,随机分为乳酸林格液复苏组(RL组)、高渗盐(7.5%)复苏组(HTS组)、羟乙基淀粉复苏组(HES组)、高渗盐联合羟乙基淀粉复苏组(HSH组),每组15只。分别测量不同时间点平均动脉压(mean arterial blood pressure,MAP),光镜下观察肠组织病理学变化,采用酶联免疫吸附法(ELISA)检测大鼠血清NF-κB含量,免疫组化检测肠组织ICAM-1,末端脱氧核苷酸转移酶介导的原位末端标记法(TUNEL)检测检测小肠黏膜细胞凋亡情况。结果不同液体复苏完毕时HTS组MAP均小于RL组HES组和HSH组(P0.05)。病理切片HE染色示在保护小肠粘膜损伤方面RL组和HES组显著低于HTS和HSH组(P0.01)。ICAM-1和nf-κb表达测定HTS组、HSH组明显比RL组和HES组低(P0.01)。HSH组亦比HTS组ICAM-1和nf-κb表达低(P0.05)。RL组、HES组、HTS组、HSH组细胞凋亡检测两两比较均有统计学意义(P0.05)。结论HSH可明显减轻肠道黏膜的损伤,降低NF-κB及ICAM-1的表达,减少细胞凋亡,有助于更好地保护机体细胞和维持器官功能状态。
[Abstract]:Objective to investigate the effects of different means of fluid resuscitation on hemorrhagic shock in rats intestinal morphology, inflammation and immune function and cell apoptosis. Methods in this study, 60 SD rats were made into models of hemorrhagic shock were randomly divided into lactated Ringer's solution group (RL group), hypertonic saline resuscitation group (HTS (7.5%) hydroxyethyl starch group), resuscitation group (HES group), hypertonic saline hydroxyethyl starch combined with resuscitation group (HSH group), 15 rats in each group were measured at different time points. The mean arterial pressure (mean arterial blood pressure, MAP), under the light microscope observation of the intestinal pathological changes, using enzyme-linked immunosorbent assay (ELISA) detection of rat serum NF- kappa B content, immunohistochemical detection of ICAM-1 intestinal tissue, terminal deoxynucleotidyl transferase mediated dUTP nick end labeling (TUNEL) enzyme mediated detection of intestinal mucosal cell apoptosis detection. The results of different fluid resuscitation at the end of the HTS group MAP HES group and RL group were less than H SH group (P0.05). HE staining showed in the protection of intestinal mucosa injury in RL group and HES group was significantly lower than that in HTS and HSH group (P0.01) and.ICAM-1 nf- kappa B expression determination of HTS group, HSH group than in RL group and HES group (P0.01) and low.HSH group than the HTS group and the low expression of ICAM-1 the expression of nf- B (P0.05).RL group, HES group, HTS group, HSH group apoptosis was detected in 22 were statistically significant (P0.05). Conclusion HSH can alleviate the damage of intestinal mucosa, reduce the expression of NF- K B and ICAM-1, reduce apoptosis, helps to protect the body cells and maintain organ function can the state.

【学位授予单位】：石河子大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R459.7

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