基于微流控的即时检测芯片的设计与制造

发布时间：2018-04-04 19:40

本文选题：微流控　切入点：超声封合　出处：《大连理工大学》2015年硕士论文

【摘要】：即时检测(POCT)技术是一种临床医学中的快速检测技术,目前已广泛应用于血糖、肿瘤检测等各个方面。基于微流控的即时检测芯片属于微流控技术、生物医学工程、先进制造技术等的联合应用,本文围绕该芯片的研制,做了以下几个方面的研究：确定了基于微流控技术、荧光免疫检测技术的即时检测芯片方案。芯片的材料采用热塑性聚合物,结合注塑成型工艺和超声波封合技术,以实现芯片批量制造的目的。设计的芯片结构主要包括进样区、混合区、流速控制结构、检测区和废液回收区等。为验证芯片结构方案的可行性,采用微加工工艺,制作出SU-8模具,并采用浇注的方法,制作出PDMS微流控芯片,对芯片各功能区域特别是流速控制结构,进行功能性试验,最终确定了芯片设计方案。选用超声波封合作为芯片的封合方法,并设计了一种配合式的导能筋结构。合理设计了芯片的整体结构,使其有利于注塑成型的质量,以及符合芯片的定位和封合过程中的装夹要求。基于Taguchi正交试验设计方法,进行芯片的注塑成型工艺试验,获得了各工艺参数对芯片注塑成型质量的主次影响顺序和其最优水平组合,得出了各个注塑成型工艺参数对芯片微结构填充和封合面的平面度的影响规律；通过订制特定的焊头,自行设计和制作芯片夹具,搭建超声波封合实验平台。通过多次封合实验,得到超声封合最佳工作参数。以羊全血作为实验样品,进行芯片的自进样实验。实验过程中,芯片各区域都表现出较好的流动稳定性,通过15组重复实验得出,血样流动前沿流过流速控制结构的时间的标准差为8.3s,流过整个芯片的时间的标准差为19.4s。
[Abstract]:Instant detection technique is a rapid detection technique in clinical medicine, which has been widely used in blood sugar, tumor detection and so on.The real-time detection chip based on microfluidic is the joint application of microfluidic technology, biomedical engineering, advanced manufacturing technology and so on.The real-time detection chip scheme of fluorescence immunoassay technology.The chip is made of thermoplastic polymer, injection molding technology and ultrasonic sealing technology.The chip structure includes sampling area, mixing area, flow control structure, detection area and waste recovery area.In order to verify the feasibility of the chip structure, the SU-8 mould was fabricated by micromachining technology, and the PDMS microfluidic chip was fabricated by pouring method. The functional tests were carried out on the functional regions of the chip, especially the flow control structure.Finally, the chip design scheme is determined.The ultrasonic sealing cooperation is selected as the sealing method of the chip, and a kind of matching energy conduction bar structure is designed.The whole structure of the chip is designed reasonably, which is propitious to the quality of injection molding, and meets the requirements of chip positioning and clamping in the process of sealing.Based on the orthogonal design method of Taguchi, the injection molding process of the chip was tested, and the sequence of the main and secondary effects of each process parameter on the injection molding quality of the chip and its optimal level combination were obtained.The effects of various injection molding process parameters on the flatness of the microstructural filling and sealing surfaces of the chip were obtained. The ultrasonic sealing experimental platform was built by customizing the specific welding head and designing and making the chip clamp by itself.The optimum working parameters of ultrasonic sealing were obtained through several sealing experiments.The whole blood of sheep was used as the experimental sample, and the self-injection experiment of the chip was carried out.In the process of the experiment, the flow stability of each region of the chip is good. Through 15 groups of repeated experiments, the standard deviation of the time of the blood flow front flowing through the flow velocity control structure is 8.3 s, and the standard deviation of the time passing through the whole chip is 19.4s.
【学位授予单位】：大连理工大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R446;TN492

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