脓毒症患者血清诱导血管内皮细胞通透性相关因素研究

发布时间：2018-04-04 22:35

本文选题：脓毒症　切入点：人血管内皮细胞　出处：《中国临床药理学杂志》2016年12期

【摘要】：目的观察脓毒症患者血清对人血管内皮细胞单层通透性的影响。方法选择45例脓毒症患者纳入试验组,根据处理方法的不同分为试验Ⅰ组(n=32,不处理)和试验Ⅱ组[(n=23,ROCK抑制药(Y27632)],同时将40例健康志愿者分为正常Ⅰ组(n=20,不处理)和正常Ⅱ组(n=20,Y27632)。分别以20%脓毒症患者血清刺激内皮细胞6 h;测定内皮细胞单层通透性,观察内皮细胞的形态分布并检测RhoA(Ras homolog A)蛋白的表达。结果与正常组相比,试验Ⅰ组的内皮细胞通透性显著升高(P0.05),但是试验Ⅱ组预处理Y27632后,可以显著抑制内皮细胞通透性的增高(P0.05);抑制药本身(正常Ⅱ组)对内皮细胞通透性无影响。试验Ⅰ与Ⅱ组的血清可增加内皮细胞间间隙的数量,改变细胞通透性,并能有效诱导RhoA蛋白的表达。结论脓毒症患者血清能够明显诱导血管内皮细胞通透性增高;其机制与激活Rho相关蛋白激酶/Rho相关的卷曲蛋白激酶(Rho/ROCK)信号通路、破坏内皮细胞屏障功能有关。
[Abstract]:Objective To observe the effect of serum of patients with sepsis on human vascular endothelial cell monolayer permeability. Methods 45 cases of sepsis were included in the experimental group, according to the different processing methods were divided into experimental group (n=32, no treatment) and experimental group II [(n=23, ROCK inhibitor (Y27632)], and 40 healthy volunteers were divided into normal group (n=20, no treatment) and normal group (n=20, Y27632) respectively. The serum of 20% patients with sepsis stimulate endothelial cells 6 h; Determination of endothelial cell monolayer permeability, observe the morphological distribution of endothelial cells and detection of RhoA (Ras homolog A) protein expression. Results compared with the normal group, the endothelial cell permeability test group increased significantly (P0.05), but the experimental group II after pretreatment of Y27632, increased can significantly inhibit endothelial cell permeability (P0.05); inhibit the drug itself (normal group) had no effect on endothelial cell permeability test group I and II. The serum can increase the number of endothelial cell clearance, change cell permeability, and can effectively induce RhoA protein expression. Conclusion the serum of patients with sepsis induced vascular endothelial cell permeability; its mechanism is associated with activation of Rho related protein kinase /Rho protein kinase (Rho/ROCK) coiled signal pathway related to the destruction of endothelial cells barrier function.

【作者单位】：上海交通大学医学院附属新华医院崇明分院急诊科;上海交通大学医学院附属瑞金医院外科重症监护室;
【基金】：上海市卫生计生委基金资助项目(20124318) 上海交通大学科技发展处基金资助项目(12XJ22016) 崇明县科技发展基金资助项目(CKY2012-02)
【分类号】：R459.7

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