基于微流控芯片的尿路感染细菌鉴定及抗生素敏感性测试

发布时间：2018-04-13 19:54

本文选题：微流控芯片 + 细菌鉴定　；参考：《分析化学》2016年04期

【摘要】：发展了一种微流控芯片纸基细菌分析技术,用于多重细菌鉴定与抗生素敏感性测试。制备了阵列培养池芯片,以滤纸作为衬底固定显色培养基和抗生素。利用PVDF疏水薄膜止流阀,将尿液样品引入芯片并分隔于不同培养池。借助于培养池阵列的空间分辨力,实现多重细菌分析。根据特异性显色结果实现细菌鉴定,通过实时显色强度分析实现细菌定量,依据抑制显色反应的最低抗生素浓度确定抗生素敏感性。以3种泌尿系统感染常见病原菌(大肠杆菌、金黄色葡萄球菌和粪肠球菌)为模拟测试对象进行分析,结果表明,芯片方法可以在18 h内实现对3种细菌的同时鉴定及6种抗生素敏感性测试。对照实验显示,芯片法与传统方法细菌鉴定和抗生素敏感性测试结果一致性分别为94.1%和93.9%。本研究建立的微流控芯片细菌分析方法简便快速,非常适合于医疗资源匮乏条件下的细菌分析。
[Abstract]:A microfluidic chip paper-based bacterial analysis technique was developed to identify multiple bacteria and test antibiotic sensitivity.The array culture cell chip was prepared, and the filter paper was used as the substrate to fix the color medium and antibiotics.PVDF hydrophobic film stop valve was used to introduce urine samples into the microarray and separate them in different culture cells.With the help of spatial resolution of culture cell array, multiple bacterial analysis was realized.The bacteria were identified according to the specific color reaction results, and the bacterial sensitivity was determined by real-time color intensity analysis and the minimum concentration of antibiotics to inhibit the color reaction.Three common pathogens of urinary tract infection (E. coli, Staphylococcus aureus and Enterococcus faecalis) were analyzed.The microarray method can be used to simultaneously identify three bacteria and test the sensitivity of 6 antibiotics within 18 hours.The results of contrast experiment showed that the results of microarray method and traditional method were 94.1% and 93.9%, respectively, and the results of antibiotic sensitivity test were 94.1% and 93.9% respectively.The microfluidic chip microfluidic microanalysis method established in this study is simple and rapid, and it is very suitable for bacterial analysis under the condition of lack of medical resources.
【作者单位】：广州医科大学附属广州市第一人民医院检验医学研究室;广东省临床分子医学及分子诊断重点实验室;
【基金】：国家自然科学基金(Nos.81371649,81271730,81501831) 广东省自然基金(No.2014A030313677) 广州市科技计划项目(No.2010U1-E00681)资助~~
【分类号】：R446.5

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