基于碳点“开关”型荧光探针在组氨酸检测中的研究

发布时间：2018-04-19 19:37

  本文选题：荧光碳点 + Ru　； 参考：《光谱学与光谱分析》2016年04期

【摘要】：研究并构建了一种基于荧光碳点(carbon dots,CDs)的新型"开关"探针,利用CDs荧光强度的变化进行痕量组氨酸(histidine,His)的检测。在pH 7.6的溶液中,钌(Ru)能与CDs通过静电吸引作用形成弱的基态化合物,导致CDs的荧光猝灭,此时体系处于"关闭"状态。而His与Ru有更强的亲和力,故向体系中加入His后,Ru从CDs表面竞争中下来,CDs的荧光得到恢复,此时体系被"打开"。考察了pH、缓冲溶液、反应时间、温度等因素对体系的影响,并初步讨论了Ru对CDs荧光的猝灭类型,得出了Ru对CDs荧光的猝灭属于静态猝灭的结论。实验结果表明,在pH 7.6的溶液中,在20~25℃条件下,体系在10min内反应完成。His的浓度与碳点荧光的恢复程度呈良好的线性关系,其线性范围为:6.5~219.3×10~(-6) mol·L~(-1),检出限为:2.15×10~(-6 )mol·L~(-1)。将方法应用于实际样品氨基酸注射液中组氨酸含量的测定,其RSD≤2.07%,回收率在95.7%~102.4%之间。将具有优良光学特性的CDs应用于构建"开关"型荧光探针,不但拓宽了CDs的应用范围,为一些重要物质的检测提供了参考。
[Abstract]:A new type of "switch" probe based on fluorescent carbon dots (CDs) was constructed for the detection of trace histidine (histidine) by using the change of fluorescence intensity of CDs. In the solution of pH 7.6, ruthenium Ru) can react with CDs to form a weak ground state compound through electrostatic attraction, which leads to fluorescence quenching of CDs, and the system is in a "closed" state. However, His has stronger affinity with Ru, so the fluorescence of Ru from CDs surface competition is recovered after adding His to the system, and the system is "opened". The effects of pH, buffer solution, reaction time and temperature on the system were investigated. The quenching type of CDs fluorescence by Ru was preliminarily discussed. It was concluded that the quenching of CDs fluorescence by Ru was static. The experimental results show that in pH 7.6 solution, at 20 鈩，

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