抗核抗体筛查试验与抗核抗体谱检测的相关性分析

发布时间：2018-04-25 17:41

本文选题：抗核抗体 + 间接免疫荧光　；参考：《免疫学杂志》2016年08期

【摘要】：目的通过对本实验室开展的抗核抗体(ANA)和特异性抗核抗体谱(ANAs)的分析研究,探讨二者之间的相关性。方法采用间接免疫荧光法(IIF)检测ANA,免疫印迹法检测ANAs,对1 048例ANA阳性标本进行2种方法的比对分析。结果 1 048例ANA阳性标本中,核颗粒型占44.9%;核均质型占23.3%;胞浆颗粒型占11.8%;着丝点型占8.9%;核仁型占4.3%;高尔基体型占2.3%;核周型占1.5%;中心粒型占0.9%;核点型占1.1%;胞浆纤维型占0.9%。主要核型为核颗粒型和核均质型。ANAs的阳性率为60%。所有荧光核型均以抗SS-A抗体阳性为多,其中n RNP/Sm、Sm、SS-A、SS-B、Ro-52特异性抗体的荧光核型以核颗粒型为主;抗nukleosome、his-tones抗体的核型主要是核均质型;胞浆颗粒型中的主要特异性抗体为n RNP/Sm、SS-A、Ro-52;抗CENP B对着丝点核型的诊断具有较高的特异性。结论 ANA检测的荧光核型与ANAs特异性抗体之间有一定的相关性,但仍存在不一致性。必须联合检测才能提高检出率,在AID的诊断中发挥一定的互补作用。
[Abstract]:Objective to study the correlation between antinuclear antibody (Ana) and specific antinuclear antibody (ANAS) in our laboratory. Methods Ana was detected by indirect immunofluorescence assay (IIFI), and ANAs was detected by immunoblotting. The two methods were compared with each other in 1 048 ANA positive specimens. Results in 1 048 ANA positive specimens, nuclear granular type accounted for 44.9%, nuclear homogeneous type accounted for 23.3T, cytoplasmic type 11.8%, metacentric type 8.9%, nucleolar type 4.3%, Golgi body type 2.3%, perinuclear type 1.5%, central granulocyte type 0.9%, nuclear point type 1.1%, cytoplasmic fiber type 0.9%. The positive rate of nuclear granular type and nuclear homogeneous type. Anas was 60%. Most of the fluorescent karyotypes were positive for anti SS-A antibodies. The fluorescent karyotypes of the specific antibodies against nRNPP / SmSS-SS-SS-BnRo-52 were mainly nuclear granules, and the karyotypes of anti-nukleosometones antibodies were mainly nuclear homoplasmas, and those of anti-nukleosomethome-his-tones antibodies were mainly nuclear homotypic. The main specific antibody in cytoplasmic granular type was nRNP / SmSS-AS-Ro-52.Anti CENP B was highly specific for the diagnosis of centromere karyotype. Conclusion there is some correlation between fluorescent karyotype detected by ANA and ANAs specific antibody, but there is still inconsistency. In order to improve the detection rate, combined detection must play a complementary role in the diagnosis of AID.
【作者单位】：甘肃省人民医院检验中心;
【分类号】：R446.61

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