临床分离大肠埃希菌多重耐药菌株与外膜蛋白的相关研究

发布时间：2018-04-29 21:38

本文选题：大肠埃希菌 + 多重耐药　；参考：《中国抗生素杂志》2016年05期

【摘要】：目的了解临床分离大肠埃希菌对临床常用抗菌药物的耐药情况,并探讨其外膜蛋白与耐药性的相关性。方法收集2013年全年宁夏医科大学总医院及其分院患者标本中分离的大肠埃希菌共1058株,统计分析其标本来源和科室构成,纸片扩散法测定6种常用抗菌药物(头孢曲松、氨曲南、左氧氟沙星、环丙沙星、庆大霉素、阿米卡星)的药敏性,荧光定量PCR法检测Omp A、Omp F、Omp C m RNA表达水平,蛋白垂直电泳检测Omp A、Omp F和Omp C蛋白的表达水平。结果 1058株大肠埃希菌耐药情况为头孢曲松(67%)、环丙沙星(65.9%)、左氧氟沙星(61.9%)、庆大霉素(50.3%)、氨曲南(48.1%)、阿米卡星(3.7%)、单药耐药(31.0%)、双药耐药(30.9%)和多重耐药(27.9%),在多重耐药株中呈现Omp C m RNA和Omp C蛋白的高表达。结论大肠埃希菌外膜蛋白Omp C表达的强弱与细菌耐药有关,可能是导致细菌耐药的原因之一。
[Abstract]:Objective to investigate the resistance of clinical isolates of Escherichia coli to common antimicrobial agents and to explore the correlation between the outer membrane protein and drug resistance. Methods A total of 1058 strains of Escherichia coli were collected from the patients of Ningxia Medical University General Hospital and its branches in 2013. The samples were collected and analyzed. Six common antimicrobial agents (ceftriaxone) were determined by disk diffusion method. The drug sensitivity of aztreonam, levofloxacin, ciprofloxacin, gentamicin and amikacin were detected by fluorescence quantitative PCR assay. The expression of Omp Aomp F and Omp C protein were detected by vertical electrophoresis. Results the drug resistance of 1058 strains of Escherichia coli were ceftriaxone 67, ciprofloxacin 65.9am, levofloxacin 61.9, gentamicin 50.33, amicacin 3.7m, single drug resistance 31.0%, double drug resistance 30.9%, and multidrug resistance 27.990%. Overexpression of Omp C m RNA and Omp C protein. Conclusion the expression of Omp C of Escherichia coli outer membrane protein is related to bacterial drug resistance, which may be one of the causes of bacterial drug resistance.
【作者单位】：宁夏医科大学总医院医学实验中心;宁夏临床病原微生物重点实验室;宁夏医科大学检验学院;
【基金】：宁夏医科大学校级课题面上项目(No.XM201322)
【分类号】：R446.5

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