载介孔二氧化硅纳米囊脂质体增强高强度聚焦超声对肿瘤细胞的杀伤力

发布时间：2018-04-30 06:50

本文选题：肿瘤细胞 + 培养的　；参考：《中国医学影像学杂志》2016年05期

【摘要】：目的制备载介孔二氧化硅纳米囊脂质体(MSN-LIPO),评价MSN-LIPO增强高强度聚焦超声(HIFU)对体外培养的肿瘤细胞的杀伤力。材料与方法采用薄膜水化法制备MSN-LIPO,用纳米粒度、电位分析仪和透射电子显微镜检测MSN-LIPO的基本表征;用CCK-8试验评估脂质浓度分别为0、0.1、0.2、0.5、1.0 mg/ml MSN-LIPO对U87 MG细胞的毒性;将FITC标记的MSN-LIPO与U87 MG细胞分别孵育0 min、15 min、30 min、1 h、2 h,使用共聚焦激光显微镜验证U87 MG细胞对不同分组MSN-LIPO的摄取;分别设立对照组、MSN-LIPO组、HIFU组和HIFU+MSN-LIPO组,通过流式细胞仪评价不同分组对U87 MG细胞的杀伤作用。结果制备的MSN-LIPO粒径大小约为(128.60±2.11)nm,Zeta电位约为(-22.90±0.77)m V;不同浓度组MSN-LIPO对U87 MG细胞无显著毒性作用,组间差异无统计学意义(F=1.56,P0.05);U87 MG恶性胶质瘤细胞能够成功摄取MSNLIPO,不同时间分组组间差异有统计学意义(F=675.81,P0.05);不同治疗分组对U87 MG细胞具有不同程度的杀伤作用,组间差异有统计学意义(F=713.52,P0.05)。结论本研究制备的MSN-LIPO具有良好的生物相容性,可以协同增强HIFU对肿瘤细胞的杀伤力,具有潜在的临床应用价值。
[Abstract]:Objective to prepare a mesoporous silica nanocyst liposome (MSN-LIPOO) and evaluate the cytotoxicity of MSN-LIPO enhanced high intensity focused ultrasound (HIFU) on tumor cells in vitro. Materials and methods MSN-LIPO was prepared by membrane hydration method. The basic characterization of MSN-LIPO was detected by nano-particle size, potential analyzer and transmission electron microscope, and the toxicity of lipid concentration was evaluated by CCK-8 test to U87 MG cells. FITC labeled MSN-LIPO and U87 MG cells were incubated for 0 min, 15 min, 30 min and 1 h for 2 h, respectively. The uptake of MSN-LIPO in different groups of U87 MG cells was examined by confocal laser microscope. The killing effect of different groups on U87 MG cells was evaluated by flow cytometry. Results the particle size of MSN-LIPO was about 128.60 卤2.11nmGV, the Zeta potential was about -22.90 卤0.77mV.The MSN-LIPO of different concentrations had no significant toxic effect on U87 MG cells. There was no significant difference between the two groups. There was no significant difference in the uptake of MSNLIPO by the malignant glioma cells of FNLIPO at different time groups, and there was a significant difference between different treatment groups in killing U87 MG cells in different treatment groups, and there was a significant difference between the two groups in the killing activity of U87 MG cells. There was a significant difference between the two groups in the treatment of U87 MG cells, and there was a significant difference between the two groups in the uptake of MSNLIPO, and there was a significant difference between different treatment groups in the killing effect of U87 MG cells. Conclusion the MSN-LIPO prepared in this study has good biocompatibility and can synergistically enhance the cytotoxicity of HIFU to tumor cells and has potential clinical application value.
【作者单位】：第二军医大学研究生院;中国科学院深圳先进技术研究院生物医学与健康工程研究所保罗.C.劳特伯生物医学成像研究中心;广州军区广州总医院超声科;
【基金】：国家自然科学基金项目(81371563) 全军医学科研“十二五”计划课题(CWS12J076) 广东省科技计划项目(2011B080701019,2012B031800309,2014A020212255)
【分类号】：R454.3;R730.5

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