耐多药结核分枝杆菌基因位点表达研究

发布时间：2018-05-15 10:41

本文选题：耐多药结核分枝杆菌 + 耐药基因　；参考：《实用医学杂志》2016年24期

【摘要】：目的:了解临床分离的耐多药结核分枝杆菌菌株耐药基因的突变情况。方法:采用基因芯片法对耐多药结核分枝杆菌临床分离菌株的耐药基因进行检测,研究其耐药基因突变情况。结果:80株耐多药结核分枝杆菌临床分离株中未见单一耐药情况,全部耐异烟肼(isoniazid,INH)+利福平(rifampicin,RFP),其中耐异烟肼+利福平+链霉素(streptomycin,SM)+乙胺丁醇(ethambutol,EMB)的耐药率明显高于其他耐药情况,差异有统计学意义(P0.05)。rpoB基因突变位点主要为第S531L、D516G、H526Y、D516V、H526D位密码子,其中第S531L和D516G位密码子突变率分别为72.5%和75.0%,明显高于其他密码子,差异有统计学意义(P0.05)。katG基因突变位点主要为第315M位密码子,突变率为75%。inhA基因突变位点主要为第-15M位密码子,突变率为87.5%。rpsL基因突变位点主要为第43M、88M位密码子,突变率分别为85.2%、14.8%。embB基因突变位点主要为第306M2位密码子,突变率为65%。结论:PCR及基因位点突变测定可快速检测耐多药结核分枝杆菌的耐药基因,结核分枝杆菌耐多药性与多个耐药基因突变相关。
[Abstract]:Objective: to understand the mutation of drug-resistant gene of clinical isolates of Mycobacterium tuberculosis. Methods: the gene chip method was used to detect the resistance genes of multi drug resistant Mycobacterium tuberculosis clinical isolates, and the mutation of drug resistance gene was studied. Results: 80 strains of multi drug resistant Mycobacterium tuberculosis were not single resistant to single resistant strains. The drug resistance was all resistant to isoniazid (isoniazid, INH) + rifampin (rifampicin, RFP), and the resistance rate of isoniazid + rifampicin + streptomycin (streptomycin, SM) + ethambutol (ethambutol, EMB) was significantly higher than that of other drug resistance, and the difference was statistically significant (P0.05).RpoB gene mutation site was mainly S531L. The mutation rates of the codons at S531L and D516G are 72.5% and 75% respectively, which are significantly higher than those of other codons. The difference is statistically significant (P0.05).KatG gene mutation site is mainly the 315M bit codon, and the mutation rate of the 75%.inhA gene is mainly the number -15M codon, and the mutation rate is the main mutation site of 87.5%.rpsL gene. The mutation rate was 85.2% and the mutation rate of the 14.8%.embB gene was 85.2%. The mutation rate of the 14.8%.embB gene was mainly the 306M2 codon, and the mutation rate was 65%. conclusion: PCR and gene site mutation assay could quickly detect the resistance genes of MTB. The multidrug resistance of Mycobacterium tuberculosis was related to multiple resistance gene mutations.

【作者单位】：安徽省立医院感染病院检验科;
【分类号】：R440

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