短波紫外线灭活血小板中病毒的实验研究

发布时间：2018-05-16 12:39

本文选题：病毒灭活 + 血小板　；参考：《中国输血杂志》2016年06期

【摘要】：目的建立短波紫外线(UVC)对血小板悬液中伪狂犬病毒和辛德毕斯病毒灭活的实验方法,探讨UVC法对血小板计数的影响。方法以伪狂犬病毒和辛德毕斯病毒为试验病毒,Vero细胞为培养细胞,用病毒感染细胞,制备病毒增殖液;用SSP+盐溶液替代血浆重悬血小板;含不同浓度核黄素的血小板SSP+悬液分别以不同辐照剂量的UVC照射,通过细胞感染试验评价灭活效果,筛选最佳灭活条件。用血液细胞分析仪测定UVC处理前后血小板的计数。结果短波紫外线对血小板病毒灭活作用显著,单面辐照强度为4.32 mw/cm2,照射30 s能使血小板SSP+悬液中伪狂犬病毒和辛德毕斯病毒滴度均下降超过4个对数级;照射1 min,伪狂犬病毒全部灭活(下降5.38个对数级),辛德毕斯病毒全部灭活(下降4.59个对数级),UVC处理后血小板计数明显下降,P0.05;UVC联合核黄素,在相同的照射时间下,添加核黄素组与单独使用UVC组对病毒滴度降低不明显,差异无统计学意义。在辐照剂量相同的条件下,添加核黄素组血小板计数降低较无核黄素组降低缓慢,两两比较差异有统计学意义(P0.05)。结论 UVC法可有效灭活血小板中伪狂犬病毒和辛德毕斯病毒;SSP+盐溶液替代血浆重悬血小板进行UVC照射,能大大缩短病毒灭活时间,提升灭活效果;UVC联合添加核黄素对病毒灭活没有明显的协同作用,但提示对血小板有一定的保护作用。
[Abstract]:Objective to establish a method for inactivation of pseudorabies virus and Sindbeth virus in platelet suspension by short wave ultraviolet C (UV C) and to explore the effect of UVC method on platelet count. Methods Vero cells of pseudorabies virus and Sindbeth virus were used as culture cells, virus proliferative fluid was prepared by virus infection, and SSP salt solution was used instead of plasma heavy suspension platelets. Platelet SSP suspensions with different concentrations of riboflavin were irradiated with different doses of UVC. The inactivation effect was evaluated by cell infection test and the best inactivation conditions were screened. The platelet count before and after UVC treatment was measured by blood cell analyzer. Results there was significant inactivation of platelet virus by short-wave ultraviolet radiation. The intensity of single side irradiation was 4.32 mw / cm ~ 2. The titers of pseudorabies virus and Sindbeth virus in platelet SSP suspension decreased by more than 4 logarithmic grades after 30 seconds irradiation. After irradiation for 1 min, pseudorabies virus was completely inactivated (down 5.38 logarithmic order, Sindbeth virus was completely inactivated (4.59 logarithmic order UVC decreased, platelet count decreased significantly after treatment with P0.05U VC and riboflavin, and at the same irradiation time), There was no significant difference between riboflavin group and UVC group. Under the same irradiation dose, the platelet count of riboflavin group decreased more slowly than that of no riboflavin group, and the difference was statistically significant (P 0.05). Conclusion UVC method can effectively inactivate pseudorabies virus and SSP salt solution of Sindbis virus instead of plasma heavy suspension platelets for UVC irradiation, which can greatly shorten the time of virus inactivation. The combination of UVC and riboflavin had no synergistic effect on virus inactivation, but it suggested that UVC could protect platelets.
【作者单位】：广州中医药大学;广州军区广州总医院;
【基金】：国家科技重大专项课题(2011ZXJ09104-07C)
【分类号】：R457.1

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