干细胞转化技术是干细胞基础和未来临床移植研究的基石

发布时间：2018-05-18 18:22

本文选题：干细胞 + 转化型研究　；参考：《中国组织工程研究》2016年01期

【摘要】：背景:干细胞转化技术研究已成为干细胞研究的一个活跃领域,对干细胞基础研究及临床研究均产生巨大的推动作用。目的:对干细胞转化技术研究现状及进展做一综述。方法:在标题和摘要中,以"Stem cells,Transformation,differentiation"或"干细胞,转化,分化"为主题检索词,检索相关期刊论文(CNKI)、PubM ed数据库中2000年1月至2015年4月关于干细胞分化技术的文章。优先选择近期发有或发表在权威杂志上的文章。初检得到121篇文章,根据纳入标准,选择其中的64篇进行综述。结果与结论:干细胞转化技术近些年来取得了较大的进展,包括改进培养液的配比,增加特定的诱导因子、蛋白质、酶、受体,注射特定的基因,使用新氧化剂,改善培养微环境,在低氧环境下培养,使用支架,采用转基因技术,三维球形培养,共培养等。干细胞转化新技术的诞生和使用,显著提高了干细胞的转化率,为人类难治性疾病的治疗带来希望。
[Abstract]:Background: stem cell transformation technology has become an active field of stem cell research, which has a great impact on basic stem cell research and clinical research. Objective: to review the present situation and progress of stem cell transformation technology. Methods: in the title and abstract, using "Stem cells transformation differentiation" or "stem cell, transformation, differentiation" as the subject key words, the article on stem cell differentiation technology in CNKI / PubMed database was searched from January 2000 to April 2015. Preference is given to articles recently published or published in authoritative magazines. 121 articles were collected and 64 of them were reviewed according to the inclusion criteria. Results & conclusion: stem cell transformation technology has made great progress in recent years, including improving the proportion of culture medium, increasing specific inducible factors, proteins, enzymes, receptors, injecting specific genes, using new oxidants, The microenvironment was improved, cultured in hypoxic environment, scaffold, transgenic technology, three-dimensional spherical culture, co-culture and so on. The birth and application of the new technology of stem cell transformation has significantly increased the transformation rate of stem cells and brought hope for the treatment of human refractory diseases.
【作者单位】：北京脑血管病医院神经科;
【分类号】：R457.7

【共引文献】