人源Mur血型抗体的鉴定和应用——附1例报告

发布时间：2018-06-01 14:56

本文选题：血型 + 抗-Mur　；参考：《中国输血杂志》2016年12期

【摘要】：目的对1名AB型献血者血液中的抗-Mur抗体系统地进行特异性、反应能力等检测,并利用此抗体对中山市人群Mur抗原发生频率进行调查。方法在本实验室,应用合成的Mur抗原特异性多肽,通过红细胞多肽凝集抑制试验鉴定抗-Mur;英国国际血型参比实验室对该抗体进行了经典血型血清学试验、试管凝集方法和Miltenberger抗原谱细胞鉴定抗-Mur。观察蛋白水解酶、酸碱度对Mur抗原抗体反应的影响,以微量板盐水法检测广东中山地区Mur抗原发生频率。结果确定了该抗体是高质量的Mur血型抗原检测试剂;木瓜酶、菠萝酶能破坏Mur抗原,弱酸性环境能增强Mur抗原抗体反应,中山地区献血者Mur抗原占7.31%(128/1 752)。结论我们发现的这例人源抗-Mur,无ABO天然抗体存在,效价高,特异性强,为理想的Mur血型定型抗体,为今后国内外输血界对Mur血型相关研究提供了基本保证。中山地区Mur抗原发生频率较高,输血时应该予以充分的重视。
[Abstract]:Objective to detect the specificity and reaction ability of anti--Mur antibody in blood of a blood donor of type AB, and to investigate the frequency of Mur antigen occurrence in Zhongshan city. Methods in our laboratory, we used the synthetic Mur antigen-specific polypeptide to identify anti-Murr by erythrocyte polypeptide agglutination inhibition test. Anti-Murr was identified by tube agglutination method and Miltenberger antigen spectrum. The effects of proteolytic enzyme and pH on the reaction of Mur antigen and antibody were observed. The frequency of Mur antigen occurrence in Zhongshan area of Guangdong province was detected by microplate saline method. The results showed that the antibody was a high quality reagent for detecting Mur blood group antigen, papain and pineapple enzyme could destroy Mur antigen, weak acid environment could enhance Mur antigen-antibody reaction, Mur antigen of blood donors in Zhongshan area accounted for 7.31 / 1 752%. Conclusion this case of human anti-Murr has no ABO natural antibody, has high titer and strong specificity, which provides a basic guarantee for the future study of Mur blood group in the field of blood transfusion at home and abroad. The frequency of Mur antigen is high in Zhongshan area, and should be paid more attention to when transfusion.
【作者单位】：中山市中心血站;中国科学院苏州生物医学工程技术研究所;
【基金】：中山市科技计划项目(2014A1FC045) 江苏省博士后科研资助计划(1501136B) 江苏省自然科学基金(BK20130343)
【分类号】：R446.6;R457.11

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