血液样本处理方式对Wistar大鼠血液生化指标检测结果的影响

发布时间：2018-06-02 04:22

本文选题：Wistar大鼠 + 血清　；参考：《中国比较医学杂志》2016年11期

【摘要】：目的观察血液样本处理方式对大鼠血液生化指标检测结果的影响。方法取体重180~220 g的SPF级Wistar大鼠40只,雌雄各半,禁食不禁水12 h后,用3%戊巴比妥钠麻醉后取全血和肝素抗凝血各3 m L。将每种血样分别置于3个1.5 m L的离心管和肝素抗凝管中各1 m L,并分别在室温放置1 h、2 h、4 h后进行离心,测定血糖、血脂、蛋白、肝功能、肾功能、心肌酶等指标。结果血液样本类型和采集后离心前的放置时间对Wistar大鼠的血清或血浆TC、TG、TP、ALB、ALT、ALP、BUN和CREA等指标均无明显影响(P0.05)。但血浆的GLU值显著高于血清(P0.05,P0.01),并随着采集后离心前放置时间的延长持续下降。血清AST和CK、LDH值则均显著高于血浆(P0.05,P0.01),且随采集后离心前放置的延长呈现持续显著的上升(P0.05,P0.01)。而采集后离心前放置时间对Wistar大鼠血浆AST和CK、LDH值均无明显影响(P0.05)。结论血液样本类型对血脂、蛋白和肾功能指标无明显影响,但对GLU、AST、CK、LDH等检测结果有一定影响。采集后离心前的放置时间延长可导致血清血浆GLU下降,血清AST、CK、LDH升高,但对血浆AST、CK、LDH指标无明显影响。
[Abstract]:Objective to observe the effect of blood sample treatment on blood biochemical indexes in rats. Methods Forty SPF grade Wistar rats weighing 180 ~ 220g, male and female, were selected. After fasting for 12 hours, the whole blood and heparin anticoagulant blood were taken after anaesthesia with 3% pentobarbital sodium. Each blood sample was placed in 3 1.5 mL centrifuge tubes and 1 mL heparin anticoagulant tube respectively. The blood glucose, blood lipid, protein, liver function, renal function and myocardial enzyme were measured by centrifugation at room temperature for 1 h and 2 h for 4 h. Results the type of blood sample and the time before centrifugation had no significant effect on the serum or plasma TCCT TGV, alt, ALP, bun and CREA of Wistar rats. However, the GLU value of plasma was significantly higher than that of serum P0.05, P0.01C, and decreased with the prolongation of the time before centrifugation. The values of serum AST and CKD were significantly higher than that of plasma P0.05U P0.01g, and the values of serum AST and CKD showed a continuous and significant increase with the prolongation of pre-centrifugation after collection (P0.05U P0.01a). However, the time before centrifugation had no significant effect on the levels of plasma AST and CKD in Wistar rats. Conclusion Blood sample type has no significant effect on blood lipid, protein and renal function, but it has some influence on the detection results of GLU ASTX CKD and LDH. The prolongation of the storage time before centrifugation resulted in the decrease of plasma GLU and the increase of serum ASTCKN LDH, but there was no significant effect on the plasma ASTK GLU index.
【作者单位】：浙江中医药大学动物实验研究中心;
【基金】：浙江省教育厅科研项目(Y201329481) 浙江中医药大学比较医学创新团队(XTD201301)
【分类号】：R-332;R446.11

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