大肠埃希菌与肺炎克雷伯菌中质粒介导的rmtA及rmtB甲基化酶基因检测研究

发布时间：2018-06-04 13:05

  本文选题：rmtA基因 + rmtB基因　； 参考：《中华医院感染学杂志》2016年05期

【摘要】：目的检测质粒介导的16S rRNA甲基化酶基因rmtA和rmtB,在临床分离的产超广谱β-内酰胺酶(ESBLs)或耐阿米卡星大肠埃希菌和肺炎克雷伯菌中的分布,为临床合理用药提供依据。方法细菌的鉴定和药敏试验采用VITEK-2Compact系统,ESBLs阳性细菌采用双纸片协同试验证实,采用PCR法检测rmtA和rmtB基因。结果 108株细菌均未检测到rmtA基因,肺炎克雷伯菌和大肠埃希菌中rmtB基因的检出率分别为15.4%和1.8%;对于产ESBLs菌株,12.0%的肺炎克雷伯菌包含rmtB基因而大肠埃希菌未检出;20.0%~62.5%的阿米卡星耐药菌株检测到rmtB基因,且rmtB基因阳性菌株同时对庆大霉素和妥布霉素耐药;肺炎克雷伯菌和大肠埃希菌对阿米卡星、庆大霉素及妥布霉素的耐药率分别为15.4%与8.9%、42.3%与62.5%、21.2%与28.6%。结论在肺炎克雷伯菌和大肠埃希菌临床分离株存在16S rRNA甲基化酶基因,且阿米卡星耐药菌rmtB基因阳性率相对高,所有菌株未检出rmtA基因。
[Abstract]:Objective to detect the distribution of plasmid mediated 16s rRNA methylase genes rmtA and rmtBin in extended-spectrum 尾 -lactamases (ESBLs) or Escherichia coli and Klebsiella pneumoniae. Methods the identification of bacteria and drug sensitivity test were confirmed by VITEK-2Compact system and double disk synergy test. RmtA and rmtB genes were detected by PCR method. Results rmtA gene was not detected in 108 strains of bacteria. The detection rate of rmtB gene in Klebsiella pneumoniae and Escherichia coli were 15.4% and 1.8%, respectively. RmtB gene was detected in 12.0% of Klebsiella pneumoniae strains producing ESBLs and 62.5% in Escherichia coli. The resistance rates of Klebsiella pneumoniae and Escherichia coli to amikacin, gentamicin and tobramycin were 15.4% and 8.92.3% respectively. Conclusion 16s rRNA methylase gene exists in clinical isolates of Klebsiella pneumoniae and Escherichia coli, and the positive rate of rmtB gene in amikacin resistant bacteria is relatively high. No rmtA gene was detected in all strains.
【作者单位】： 厦门大学附属第一医院暨福建医科大学教学医院检验科;
【基金】：国家自然科学基金资助项目(81302529)
【分类号】：R440
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本文编号：1977469