江苏农村地区结核杆菌基因分型及耐药性分析

发布时间：2018-06-12 21:54

本文选题：结核分枝杆菌 + 间隔区寡核苷酸分型　；参考：《现代预防医学》2016年19期

【摘要】：目的研究江苏省农村地区分离的结核分枝杆菌基因多态性,从而了解菌株基因型与耐药之间的相关性。方法采用罗氏培养基分离来自结核病患者痰标本中的结核分枝杆菌,进行间隔区寡核苷酸分型(spacer oligonucleotide typing,Spoligotyping)和比例法进行4种一线抗结核药物的药敏试验。经统计学分析,P0.05为差异具有统计学意义。结果 547株结核分枝杆菌的Spoligotyping分型结果通过与Spol DB4.0数据库比对后显示,试验菌株被分为18个簇,57个基因型,以北京基因型菌株为主,占79.89%,非北京家族菌株包括T(T1、T2)、U、H、MANU2、LAM10-CAM和CAS-DELH1等型别,各占11.15%、0.91%、0.37%、0.91%、0.18%和0.18%,以及26个型别(35株菌)为"新型"。药敏试验发现,总耐药率为21.94%,耐多药率为7.68%;单药耐药中,主要以链霉素和异烟肼耐药为主。经χ2检验和Fisher确切概率法分析发现,北京基因型与非北京基因型菌株的耐多药性方面差异具有显著统计学意义。结论江苏省农村分离的结核分枝杆菌具有明显的基因多态性,北京基因型为主要流行型,并且北京家族菌株与耐多药之间具有一定的相关性。
[Abstract]:Objective to study the genetic polymorphisms of Mycobacterium tuberculosis isolated from rural areas of Jiangsu Province, and to understand the relationship between genotype and drug resistance. Methods Mycobacterium tuberculosis isolated from sputum samples of patients with tuberculosis was isolated from Roche medium. Spacer oligonucleotide typing and drug susceptibility tests were performed for four first-line antituberculotic drugs by means of spacer oligonucleotide typing and proportion method. By statistical analysis, P0.05 for the difference has statistical significance. Results the results of Spoligotyping of 547 strains of Mycobacterium tuberculosis were compared with Spol DB 4.0 database. The results showed that the tested strains were divided into 18 clusters and 57 genotypes, the majority of which were Beijing genotypes (79.89%). Each 11.15% 0.91% 0.370.37% and 0.18% 0.18% and 0.18%, as well as 26 types of 35 strains) are "new type". The drug sensitivity test showed that the total drug resistance rate was 21.94 and the multidrug resistance rate was 7.68.The main drug resistance in single drug resistance was streptomycin and isoniazid. 蠂 2 test and Fisher exact probability analysis showed that there was significant difference in multidrug resistance between Beijing genotype and non-Beijing genotype strain. Conclusion Mycobacterium tuberculosis isolated from rural areas of Jiangsu Province has obvious gene polymorphism, Beijing genotype is the main epidemic type, and there is a certain correlation between Beijing family strains and multi-drug resistance.
【作者单位】：长治医学院免疫学教研室;中国疾病预防控制中心传染病预防控制所国家重点实验室;江苏省疾病预防控制中心;国家传染病协同创新中心;
【基金】：中国超级传染病国家“十二五”规划重点课题(2013ZX10003002-001) 山西省应用基础研究项目(201601D021161) 山西省高等学校教学改革创新项目(J2016076) 山西省教育科学“十二五”规划课题(GH14019) 山西省高等学校大学生创新创业训练计划项目(2016303) 长治医学院科技创新团队支持项目(CX201412)
【分类号】：R446.5

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