应用活细胞RNA检测纳米技术检测乳腺上皮细胞MCF-10A及乳腺癌MCF-7细胞的miR-142-3p表达

发布时间：2018-06-13 00:23

本文选题：活细胞RNA检测纳米技术 + 乳腺上皮细胞系　；参考：《中国生物化学与分子生物学报》2016年05期

【摘要】：传统的核酸检测技术如放射性核素、荧光、化学修饰的探针以及核酸扩增等技术无法检测活细胞中核酸的表达量。而活细胞RNA纳米检测技术和传统的检测技术相比,利用纳米金颗粒为探针能对活细胞进行检测,实验步骤更为简单,可以在自然的、无扩增的条件下观察RNA,这可真实地反应基因表达与表型之间的关系。miRNA是一类非编码RNA,其长度为20~24个碱基,在生命活动中起重要的作用。本文应用活细胞RNA检测纳米技术结合荧光定量PCR分别检测正常的乳腺上皮细胞系及乳腺上皮癌细胞系中内源性miR-142-3p的表达,发现乳腺癌细胞系内源性miR-142-3p的表达显著高于正常乳腺上皮细胞系中miR-142-3p的表达,结果提示miR-142-3p可能在乳腺癌细胞发生发展中起到调控作用。
[Abstract]:Traditional nucleic acid detection techniques, such as radionuclide, fluorescence, chemically modified probes and nucleic acid amplification, can not detect the expression of nucleic acid in living cells. Compared with the traditional detection technology, the living cell RNA nano-detection technology can be used as a probe to detect living cells. The experimental procedure is simpler and can be used naturally. Observation of RNAs without amplification can truly reflect the relationship between gene expression and phenotype. MiRNA is a class of noncoding RNAs with a length of 20-24 bases, which plays an important role in life activities. The expression of endogenous miR-142-3p in normal breast epithelial cell line and breast epithelial carcinoma cell line was detected by living cell RNA assay and fluorescence quantitative PCR. It was found that the expression of miR-142-3p in breast cancer cell line was significantly higher than that in normal mammary epithelial cell line, which suggested that miR-142-3p might play a regulatory role in the development of breast cancer cells.
【作者单位】：东北农业大学乳品科学教育部重点实验室;
【基金】：国家自然科学基金项目(No.31072103)~~
【分类号】：R440

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