多耐药大肠埃希菌NB8株全基因组耐药基因检测

发布时间：2018-06-16 15:05

本文选题：大肠埃希菌 + 全基因组　；参考：《中国人兽共患病学报》2016年08期

【摘要】：目的检测1株多耐药大肠埃希菌NB8株的遗传学背景。方法病原分离自2012年4月宁波市第一医院住院患者尿液,作16SrDNA和gyrA基因测序、BLASTn比对确认为大肠埃希菌,采用Illumina HiSeq与Ion Torrent PGM两种大规模并行测序仪进行全基因组分析,再行人工测序。最后NB8株和9株已完成全基因组测序的大肠埃希菌耐药基因的比较基因组学研究。结果多耐药大肠埃希菌NB8株全基因组测序得到一条推定的染色体序列,长4 550 369bp(内含14个缺口),得到一条推定的质粒序列,长635 377bp(内含33个缺口)。从NB8株全基因组数据中挖掘出了β-内酰胺类、氨基糖苷类、喹诺酮类、大环内酯类、磺胺类、四环素类、多粘菌素的耐药基因,并挖掘出接合性质粒、转座子、插入序列、整合子的遗传标记,以及5大类外排泵基因。结论多耐药大肠埃希菌NB8株遗传学背景明确,主动外排机制增强也会导致或者增强NB8株的耐药性。质粒、转座子和整合子等可移动遗传元件可以使细菌的耐药性得以快速传播,使受体菌表现为多重耐药。
[Abstract]:Objective to detect the genetic background of a multidrug resistant Escherichia coli strain NB 8. Methods the pathogens were isolated from the urine of the inpatients in the first Hospital of Ningbo City in April 2012. The results were compared with 16s rDNA and gyrA gene sequencing and identified as Escherichia coli. Two large-scale parallel sequencing instruments, Illumina HiSeq and Ion Torrent PGM, were used to analyze the whole genome of Escherichia coli. Then artificial sequencing was performed. Finally, the comparative genomics study of drug-resistant genes of Escherichia coli was carried out in Nb8 and 9 strains of Escherichia coli. Results A putative chromosome sequence was obtained by sequencing the whole genome of multidrug resistant Escherichia coli strain NB8, with a length of 4 550 369bp (containing 14 notches) and a deduced plasmid sequence with a length of 635 377bp (containing 33 gaps). 尾 -lactams, aminoglycosides, quinolones, macrolides, sulfonamides, tetracyclines, polymyxin were extracted from genomic data of Nb8 strain. The genetic markers of integron, and 5 kinds of efflux pump genes. Conclusion the genetic background of multidrug resistant Escherichia coli strain NB8 is clear, and the enhancement of active efflux mechanism will lead to or enhance the drug resistance of Nb8 strain. Transportable genetic elements such as plasmids, transposons and integrons can rapidly spread the drug resistance of bacteria and make the bacteria multidrug resistant.
【作者单位】：宁波市第一医院检验科;无锡市克隆遗传技术研究所生物信息学室;南京医科大学附属无锡人民医院医学检验科;杭州市余杭区中医院检验科;
【基金】：宁波市自然科学基金(No.2012A610186) 浙江省中医药基金(No.2011ZB126)资助~~
【分类号】：R440

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