江西省部分医院临床分离沙门菌耐药性与分子分型研究

发布时间：2018-06-20 11:24

本文选题：感染性腹泻 + 沙门菌　；参考：《中国消毒学杂志》2016年12期

【摘要】：目的研究江西省部分医院临床分离的致泻沙门菌耐药性与分子分型,为临床科学选择抗菌药物提供参考。方法采用微量肉汤稀释法和基因检测技术,对江西省部分医院临床分离的腹泻性沙门菌进行药敏试验和基因分析。结果从医院住院感染性腹泻患者送检标本中分离出沙门菌103株,对四环素耐药率达到61.17%,有27.18%的菌株呈现多重耐药性。该省多数地区医院分离的沙门菌对磺胺类、四环素类、头孢类抗菌药物耐药率均有上升趋势,对喹诺酮类抗菌药物较敏感。从2015年分离的菌株基因分型检测发现52种带型,每种带型包含菌株为1~3株,相似度为0%~100%。结论该省医院分离的腹泻感染沙门菌耐药状况比较严重,菌株PFGE带型呈现多样性,提示应加强抗菌药物管理。
[Abstract]:Objective to study the antimicrobial resistance and molecular typing of Salmonella purpureus isolated from some hospitals in Jiangxi Province, and to provide reference for the selection of antimicrobial agents in clinical science. Methods Drug sensitivity test and gene analysis of Salmonella diarrhea isolated from some hospitals in Jiangxi Province were carried out by using broth dilution method and gene detection technique. Results 103 strains of Salmonella were isolated from patients with infectious diarrhea in hospital. The resistance rate to tetracycline reached 61.17%, and 27.18% strains showed multidrug resistance. The resistance rate of Salmonella isolated from hospitals in most areas of this province to sulfadiazine tetracycline and cephalosporins showed an increasing trend and was more sensitive to quinolones. From the genotyping analysis of the strains isolated in 2015, 52 banding types were found, each of which contained 1: 3 strains, and the similarity was 0 ~ 100%. Conclusion the drug resistance of Salmonella isolated from the hospital of this province is serious, and the PFGE bands of the strains are diverse, suggesting that the management of antimicrobial agents should be strengthened.
【作者单位】：江西省疾病预防控制中心;
【基金】：江西省卫计委科研项目(20166022) 江西省科技厅科技支撑项目(20141BBG70095)
【分类号】：R446.5;R440

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