基质辅助激光解吸电离飞行时间质谱在临床微生物检验的应用进展

发布时间：2018-06-22 08:36

  本文选题：基质辅助激光解吸电离飞行时间质谱 + 微生物　； 参考：《中国老年学杂志》2016年13期

【摘要】：正基质辅助激光解吸电离飞行时间质谱(MALDI-TOF)是一种新型的质谱技术,具有灵敏度高,样品用量少,分析速度快的特点。目前,MALDI-TOF已开始应用于多项微生物的检测,为临床疾病的诊疗提供有效的指导。1 MALDI-TOF鉴定微生物的特点及优势MALDI-TOF系统由基质辅助激光解吸电离离子源(MALDI)、飞行时间质量分析器(TOF)和检测器组成,属于一种软电离技术,适用于混合物及生物大分子的测定〔1〕。其原理是利用激光照射待测物与基质形成的共结晶薄膜,通过基质吸收能量并将能量转移至待测物分子,使分子解离成离子,利用离子在
[Abstract]:Positive matrix assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF) is a new type of mass spectrometry technology with high sensitivity, small sample consumption and fast analysis speed. At present, MALDI-TOF has been applied to the detection of many microbes. To provide effective guidance for the diagnosis and treatment of clinical diseases. 1. The characteristics and advantages of MALDI-TOF in the identification of microorganisms. The MALDI-TOF system is composed of matrix assisted laser desorption ionization ion source (MALDI), time of flight quality analyzer (TOF) and detector. It belongs to a soft ionization technology. Suitable for the determination of mixtures and biomolecules (1). The principle is to use laser to irradiate the eutectic film formed between the tested object and the matrix, absorb the energy through the matrix and transfer the energy to the molecule to be tested, so that the molecule can be dissociated into ions.
【作者单位】： 吉林大学第一医院检验科;吉林大学第一医院神经肿瘤外科;
【分类号】：R446.5
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本文编号：2052250