连续5d快速动眼睡眠剥夺诱发小鼠迟发性抑郁样行为

发布时间：2018-06-22 09:59

本文选题：快速动眼睡眠剥夺 + 抑郁样行为　；参考：《南方医科大学学报》2016年05期

【摘要】：目的探讨连续长时间快速动眼睡眠剥夺(5 d)对C57BL/6J小鼠抑郁样行为和杏仁核单胺氧化酶A水平的影响。方法成年雄性C57BL/6J小鼠随机分成空白组、对照组和快速动眼睡眠剥夺组,通过多小平台水环境法建立快速动眼睡眠剥夺模型。睡眠剥夺结束后,于不同时间点采用旷场实验检测各组运动能力,强迫游泳实验和糖水偏好实验检测各组抑郁样行为。之后,取脑分离杏仁核,利用Western Blot和Real-time PCR检测各组单胺氧化酶A的表达水平。结果快速动眼睡眠剥夺后的1~3 d内,睡眠剥夺小鼠运动能力明显下降,第4天可恢复到正常水平;第7~14天强迫游泳实验显示小鼠不动时间延长;糖水偏好率也明显降低。Western blot和Real-time PCR检测发现杏仁核中单胺氧化酶A表达水平明显升高。结论连续长时间快速动眼睡眠剥夺导致小鼠迟发性抑郁样行为,该现象很可能与杏仁核单胺氧化酶水平变化有关。
[Abstract]:Objective to investigate the effects of continuous rapid eye movement sleep deprivation (5 d) on depressive behavior and amygdala monoamine oxidase A level in C57BL / 6J mice. Methods Adult male C57BL / 6J mice were randomly divided into three groups: blank group, control group and REM sleep deprivation group. At different time points after sleep deprivation, open field test was used to test the exercise ability of each group, forced swimming test and sugar water preference test were used to detect depressive behavior in each group. After that, the amygdala was isolated and the expression of monoamine oxidase A was detected by Western Blot and Real-time PCR. Results within 1 to 3 days after rapid eye movement sleep deprivation, the motor ability of sleep deprived mice decreased significantly and returned to normal level on the 4th day, and the forced swimming test on day 714 showed that the immobility time of mice was prolonged. The expression of monoamine oxidase A in amygdala was significantly increased by Western blot and Real-time PCR. Conclusion prolonged rapid eye movement sleep deprivation may lead to delayed depressive behavior in mice, which may be related to the changes of monoamine oxidase level in amygdala.
【作者单位】：南方医科大学基础医学院神经生物学教研室;
【基金】：国家自然科学基金(313711484)~~
【分类号】：R740

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