超高效液相色谱-三重四极杆质谱法同时检测人尿中5种利尿剂

发布时间：2018-06-26 18:58

本文选题：超高效液相色谱-串联质谱 + 利尿剂　；参考：《分析试验室》2016年11期

【摘要】：使用超高效液相色谱-串联质谱联用技术(UPLC-MS/MS)建立了人尿中5种利尿剂的检出方法。将尿样用同体积的5%乙酸铅水溶液沉淀蛋白质并离心后,将上清液用多反应监测(MRM)模式进行定性和定量分析。上述利尿剂1~5的检测限(LOD)在0.5~2 ng/m L之间;线性范围分别为3.0~700 ng/m L,1.5~1000 ng/m L,3.0~4000 ng/m L,6.0~5000 ng/m L和6.0~5000 ng/m L,线性良好(R20.99);在低、中和高3个浓度水平的日内和日间RSD均小于15%。
[Abstract]:A method for the detection of five diuretics in human urine was established by UPLC-MS / MS. Urine samples were precipitated with 5% lead acetate solution of the same volume and centrifuged. The supernatants were qualitatively and quantitatively analyzed by multi-reaction monitoring (MRM) model. The detection limit (LOD) of diuretic 1D was between 0.5 and 2 ng/m / L, and the linear range was 3. 0 ~ 700 ng/m / L ~ 1. 5000 ng/m / L ~ 1. 5 000 ng/m / L ~ 3. 0 ~ 4 000 ng/m / L ~ 6. 0 ~ 5 000 ng/m / L and 6. 0 ~ 5 000 ng/m / L, respectively, with a good linearity (R 20. 99), and less than 1 515 ~ 5 000 ng/m / L within and between days at low, medium and high concentrations.
【作者单位】：北京化工大学理学院;国家体育总局反兴奋剂中心;
【分类号】：R446.12;O657.63

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