广州市136株沙门菌耐药表型与分子分型研究

发布时间：2018-06-27 00:54

本文选题：沙门菌 + 耐药表型　；参考：《现代预防医学》2016年04期

【摘要】：目的研究广州市2013年腹泻患者感染的沙门菌的血清型分布、耐药情况以及分子流行特征。方法用传统血清学方法鉴定沙门菌的血清型,并用纸片扩散法进行抗菌药物敏感性试验,用脉冲场凝胶电泳(PFGE)对斯坦利沙门菌、肠炎沙门菌、鼠伤寒沙门菌和Ⅰ4,5,12:i:-沙门菌进行分子分型研究。结果 136株沙门菌被鉴定为29种血清型,数量居前三位的血清型为斯坦利沙门菌(19.9%)、肠炎沙门菌(15.4%)和鼠伤寒沙门菌(15.4%)。所有沙门菌菌株对四环素和萘啶酸耐药率最高,分别达到44.8%和39.7%,对环丙沙星的敏感率仅为19.9%;对头孢他啶、头孢噻肟和头孢曲松的耐药率均为7.4%。对3类及以上抗生素耐药的多重耐药菌有56(41.2%)株。27株斯坦利沙门菌和21株肠炎沙门菌分别可分为25个PFGE型和15个PFGE型。结论在广州市引起腹泻的沙门菌主要以斯坦利沙门菌、肠炎沙门菌和鼠伤寒沙门菌为主,分成多种PFGE型,呈高度散发。本地区沙门菌菌株的多重耐药现象严重,对于氟喹诺酮类和头孢类药物的耐药性升高。
[Abstract]:Objective to study the distribution of serotype, drug resistance and molecular epidemiological characteristics of Salmonella infection in diarrhea patients in Guangzhou in 2013. Methods the serotype of Salmonella was identified by traditional serological method. The susceptibility test of antimicrobial agents was carried out by disk diffusion method. Salmonella enteritidis and Salmonella enteritidis were detected by pulsed field gel electrophoresis (PFGE). The molecular typing of Salmonella typhimurium and Salmonella typhimurium was studied. Results 136 Salmonella strains were identified as 29 serotypes. The first three serotypes were Salmonella Stanley (19.9%), Salmonella enteritis (15.4%) and Salmonella typhimurium (15.4%). The resistant rates of all Salmonella strains to tetracycline and naphthalic acid were 44.8% and 39.7%, respectively, and the sensitivity to ciprofloxacin was only 19.90.The resistance rate to ceftazidime, cefotaxime and ceftriaxone was 7.4B. There were 56 (41.2%) strains of Streptococcus enteritidis and 21 strains of Salmonella enteritidis were classified into 25 PFGE types and 15 PFGE types respectively. Conclusion Salmonella pneumoniae, Salmonella enteritis and Salmonella typhimurium are the main bacteria causing diarrhea in Guangzhou, which are divided into various PFGE types and highly distributed. The multidrug resistance of Salmonella strains was serious and the resistance to fluoroquinolones and cephalosporins increased.
【作者单位】：南方医科大学珠江医院检验医学部;佛山市顺德区第一人民医院检验科;
【基金】：国家自然科学基金项目(81101314)
【分类号】：R446.5

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